Rizidingo ofukwenza ulwelwesi:
Ulwelwesi olusetshenziselwa i-cellulose acetate electrophoresis lunezidingo eziqinile.Okokuqala, izinto zokusetshenziswa zokukhiqiza ulwelwesi, i-cellulose acetate, kumele zibe nokuhlanzeka okuphezulu.Akufanele iqukathe ukungcola okufana ne-hemicellulose, i-lignin, noma ama-ion ensimbi esindayo.Lokhu kubaluleke kakhulu ekuhlukaniseni nasekuhlaziyeni amasampula, njengoba ukuthola imiphumela emihle yokuhlola kungaba inselele ngaphandle kolwelwesi oluhlanzekile.Okwesibili, i-membrane kufanele ibe nokunamathela okufanayo, ukujiya okufanele, nokumuncwa kahle kwamanzi.Ukufeza lokhu, i-membrane kufanele iboshwe ohlangothini olulodwa, ugweme ukushaywa okuphindaphindiwe emuva naphambili.Ugqinsi olunconyiwe lolwelwesi luphakathi kuka-0.1 kuya ku-0.15 mm.Uma i-membrane ishubile kakhulu, izoba nokumunca kahle kwamanzi futhi ibangele ukuhlukana okubi.Ngakolunye uhlangothi, uma i-membrane incane kakhulu, ijwayele ukuphuka futhi ayinakho amandla adingekayo.
Ukwelashwa kwangaphambili kwe-membrane ngaphambi kwe-electrophoresis:
Njengoba kushiwo ngaphambili, i-cellulose acetate membrane inobudlelwane obuncane bamanzi uma iqhathaniswa nephepha.Ngakho-ke, kubalulekile ukuqinisekisa ukuthi ulwelwesi luqukethe inani elithile lesixazululo se-buffer ngaphambiliegijimai-electrophoresis.I-membrane kufanele ifakwe ngaphambili kusixazululo se-buffer.Indlela efanele yokucwilisa i-membrane iwukuntanta ngobumnene phezu kwesixazululo se-buffer, okuvumela ulwelwesi ukuthi lumunce kancane kancane ikhambi le-buffer kusuka phansi bese licwila esixazululweni.ukugwema amagwebu phezu kolwelwesi ukuthintaimiphumela yokuhlukana.
Ukwengeza, ngemuva kokucwiliswa kwe-membrane,okwengeziweIsixazululo se-buffer kufanele sisuswe ngobumnene kusetshenziswa iphepha lokuhlunga.Akufanele yomiswe ngokuphelele noma ibe manzi ngokweqile.Uma yome kakhulu, iphazamisa ukuhlukaniswa kwe-electrophoretic.Uma imanzi kakhulu, ingathinta ukulayishwa kwesampula, kubangele ukuthi imigqa yesampula isabalale futhi izindawo zokuqala zengxenye ngayinye zingalingani, ngaleyo ndlela kuthinte ukusebenza kahle kokuhlukanisa.
Sizobhala kabanzi ngale ndaba ngokuzayo, futhi siyakwamukela ukuphawula kwakho!
Imininingwane yokwazi nge-Beijing Liuyi Biotechnology's i-cellulose acetate membraneIthangi le-electrophoresis kanye nesicelo salo se-electrophoresis, sicela uvakashele lapha:
lUkuhlolwa kokuhlukanisa amaprotheni e-serum nge-Cellulose Acetate Membrane
lI-Cellulose Acetate Membrane Electrophoresis
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Ireferensi:I-Electrophoresis(Uhlelo Lwesibili) nguMnu. He kanye noMnu.Zhang
Isikhathi sokuthumela: May-29-2023