I-Cellulose Acetate Membrane Electrophoresis

Iyini i-Cellulose Acetate Membrane Electrophoresis?

I-Cellulose Acetate Membrane Electrophoresis iwuhlobo olulodwa lwamasu e-electrophoresis esebenzisa ulwelwesi lwe-cellulose acetate njengemidiya esekela ukuhlolwa.

I-Cellulose Acetate iwuhlobo lwe-acetate ye-cellulose ene-acetylated evela ku-hydroxyl ye-cellulose.Uma inyibilika esixazululweni sezinto eziphilayo njenge-acetone, ingambozwa kufilimu e-homogeneous nenhle enama-micro-pores.Ugqinsi lwefilimu luku-0.1-0.15mm olufanele ukuhlolwa.

Izinzuzo ze-cellulose acetate membrane

Uma kuqhathaniswa nephepha le-fliter, linalezi zinzuzo ezilandelayo:

1.Umphumela omuhle wokuhlukana.Kumasampuli amaprotheni, ulwelwesi lwe-cellulose acetate lumunca ambalwa, ngemva kokudayi, lungashintsha umbala lube umbala ongemuva.Amabhande odayi acacile kakhulu, ngakho-ke ikhuthaza ukunemba kokunqunywa komthamo.
2.Fast futhi wonge isikhathi.I-cellulose acetate membrane ine-hydyophilic encane kune-fliter paper, ngakho ukusebenza kwe-electro-osmosis kungaphansi kwe-fliter paper, futhi amandla kagesi enziwa ikakhulukazi amasampula angafinyeza isikhathi sokuhlukaniswa futhi anciphise isikhathi se-electrophoresis.Imvamisa, isikhathi se-cellulose acetate membrane electrophoresis singama-45-60minutes.Yonke inqubo ye-electrophoresis ehlanganisa ukudaya nokukhishwa kombala idinga imizuzu engu-90 kuphela.
3.Ukuzwela okuphezulu kanye namasampula amancane okufanele asetshenziswe.Idinga kuphela i-2 μL serum ku-serum protein electrophoresis.
4.Uhlelo olubanzi.Amanye amaprotheni akulula ukuwahlukanisa ephepheni le-electrophoresis, njenge-Alpha-fetoprotein, i-bacteriolytic enzyme, i-insulin, ne-histone njll. Kodwa kungcono kakhulu ukusebenzisa i-cellulose acetate membrane electrophoresis ukuze uwahlukanise.
5.Easy ukugcina kanye quantification.Ngemva kokudaya amasampula e-cellulose acetate membrane electrophoresis, cwilisa amasampula kusixazululo esixubile se-glacial acetic acid ne-ethanol ukuze wenze ipuleti elomile elibonisa ngale eligcinwa isikhathi eside.

Ngenxa yokuthi i-cellulose acetate membrane electrophoresis kulula ukuyisebenzisa, manje isetshenziswa kabanzi ekuhlaziyeni nasekuhloleni amaprotheni e-plasma, i-lipoprotein, i-glycoprotein, i-Alpha-fetoprotein, i-enzyme, i-polypeptide, i-nucleic acid nezinye i-biomacromolecule.

Ukulungiselela i-Cellulose Acetate Membrane Electrophoresis

Izinto ezisetshenziswayo zihlanganisa amandla kagesi aphansi, ithangi le-electrophoresis elivundlile.Imidiya esekelayo i-cellulose acetate membrane.

Ama-reagents:

1.pH8.6 isixazululo se-barbitol buffer: i-Diethyl barbituric acid, i-Diethyl sodium pentobarbital;
2.Ibala:Ponceau S, Trichloroacetic;
I-3.TBS/T noma i-PBS/T: 95% i-ethanol, i-glacial acetic acid;
I-4.Isixazululo Sokuhlanza: i-ethanol anhydrous, i-glacial acetic acid.

I-Beijing Liuyi Biotechnology Co., ltd inohlelo oluvuthiwe lwe-cellulose acetate membrane electrophoresis.Imodeli ye-DYCP-38C iyigumbi elithambile le-electrophoresis yephepha nesilayidi, kanye ne-cellulose acetate membrane electrophoresis.Umzimba oyinhloko ubunjiwe, akukho mkhuba wokuvuza.Imodeli yokuphakelwa kwamandla i-DYY-2C, i-DYY-6C, i-DYY-6D, i-DYY-8C , i-DYY-10C ne-DYY-12 inganikeza amandla e-DYCP-38C.Ngokuvamile, ikhasimende lithanda ukukhetha imodeli ye-DYY-6C njengomthombo wamandla we-DYCP-38C.

Njengomkhiqizo odingekayo wethangi le-DYCP-38C electrophoresis, i-Liuyi iphinde inikeze ulwelwesi lwe-cellulose acetate.Kukhona osayizi abathathu abavamile be-membrane, 7 * 9cm, 2 * 8cm no-12 * 8cm, futhi singahlinzeka ngosayizi owenziwe ngezifiso we-cellulose acetate membrane njengezidingo zakho futhi.

Amanothi mayelana nokuhlolwa:

1.Layisha isampula ohlangothini olunzima:1-2UL ifanelekile
2.I-electrophoresis yamanje eqhubekayo: amandla amanje 0.4–0.6m A/cm
3.pH8.6 isixazululo sebhafa ye-barbitol:Amandla e-Ionic 0.06
4.Isikhathi sokudaya: imizuzu emi-5 eyanele
I-5.Ukugcinwa: faka imephu ye-elecrtophoresis eyomile ekuhlanzeni isisombululo semizuzu engu-10-15, bese uyikhipha bese uyinamathisela engilazini ehlanzekile, ngemva kokuba isomile, izoba imephu yefilimu ebonakalayo.

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