I-SDS-PAGE Gel Electrophoresis System

Incazelo emfushane:

I-Electrophoresis iyindlela yaselabhorethri esebenzisa amandla kagesi ukuze ahlukanise i-DNA, i-RNA noma amaprotheni ngokusekelwe ezintweni zawo ezibonakalayo njengosayizi neshaji. I-DYCZ-24DN iyiseli elincane eliqondile le-electrophoresis elingasetshenziselwa i-SDS-PAGE ijeli electrophoresis. I-SDS-PAGE, igama eligcwele yi-sodium dodecyl sulfate–polyacrylamide gel electrophoresis, evame ukusetshenziswa njengendlela yokuhlukanisa amaprotheni anenqwaba yamangqamuzana phakathi kuka-5 no-250 kDa. Kuyindlela esetshenziswa kakhulu ku-biochemistry, i-molecular biology kanye ne-biotechnology ukuhlukanisa amaprotheni ngokusekelwe esisindweni samangqamuzana awo.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ukucaciswa

Ukucaciswa Kwezobuchwepheshe Kwe-DYCZ-24DN
Ubukhulu (LxWxH)	140×100×150mm
Usayizi weGel (LxW)	75 × 83mm
Ikama	Imithombo eyi-10 nemithombo eyi-15
Ukujiya Kwekama	1.0mm kanye 1.5mm(Okujwayelekile）0.75mm (Uma uthanda)
Inombolo Yamasampuli	20-30
Ivolumu ye-Buffer	400 ml
Isisindo	1.0kg
Ukucaciswa kobuchwepheshe kwe-DYY-6C
Ubukhulu (LxWxH)	315 x 290x 128mm
I-Voltage ephumayo	6-600V
Okukhiphayo Kwamanje	4-400mA
Amandla Okukhiphayo	240W
Itheminali yokuphumayo	4 amapheya ngokuhambisana
Isisindo	5.0kg

Incazelo

I-DYCZ–24DN iyiseli elincane le-electrophoresis elima mpo kabili elincane elisetshenziselwa iphrotheni electrophoresis, okuwuhlelo oluthambile, olulula futhi olusebenziseka kalula. Ikhiqizwa nge-polycarbonate ephezulu ngama-electrode eplatinamu. Uhlelo luqukethe umzimba wethangi eliyinhloko (indawo yokuphonsa ijeli), isivalo esinomthofu, ithange langaphandle (ithangi eliyisigcinalwazi) kanye nedivayisi yokuphonsa ijeli. Izesekeli: ipuleti lengilazi, ikamu, ibhodi lengilazi eliwugqinsi (∮=5 mm) lokusebenzisa ijeli eyodwa, uhlaka olukhethekile lweweji. Ihlomisa amakamu emithombo ayi-10 no-15 anogqinsi luka-1.0mm no-1.5mm, futhi ihlinzeka ngekama ongazikhethela lona elinogqinsi oluwu-0.75mm kanye nepuleti lengilazi elinophawu elinamathiselwe ngo-regula (0.75 mm) olikhethayo. Isisekelo sayo esisobala esingenamthungo futhi esibunjwe ngomjovo sivimbela ukuvuza nokuphuka. Ingagcina isisombululo se-buffer, isisombululo se-buffer esiyisisekelo singaba ngu-170 ml; 170 ml kuphela wekhambi lebhafa ongaqedela ukuhlola. Lolu hlelo luphephe kakhulu kubasebenzisi. Umthombo wawo wamandla uzocishwa uma umsebenzisi evula isivalo. Idizayini yesivalo esikhethekile igwema ukwenza amaphutha.

I-DYY-6C iwumthombo wamandla oklanyelwe i-electrophoresis ukudala amandla kagesi ukuze anike amandla ukuhlukaniswa kwe-DNA/RNA, i-PAGE electrophoresis futhi idluliselwe kulwelwesi. I-DYY-6C isekela okukhiphayo okungu-400V, 400mA, kanye no-240W. I-LCD yayo ingabonisa i-voltage, yamanje, amandla kanye nesikhathi sesikhathi ngesikhathi esisodwa. Ingasebenza esimweni esingaguquki se-voltage, noma esimweni esingaguquki samanje kagesi, futhi iguqule ngokuzenzakalelayo ngokuvumelana nemingcele eyabelwe ngaphambilini yezidingo ezahlukene.

Isicelo

I-DYCZ-24DN enamandla okuphakela i-DYY-6C iwuhlelo lwe-electrophoresis lwe-SDS-PAGE noma i-Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis yokuhlukaniswa kwamaprotheni futhi isetshenziswa kabanzi kuma-forensics, i-genetics, i-biotechnology kanye nezindawo zebhayoloji yamangqamuzana. Abacwaningi bafingqa lezi zicelo ezilandelayo ze-SDS-PAGE:
1.Isetshenziselwa ukukala isisindo samangqamuzana ama-molecule.
2.Isetshenziselwa ukulinganisa ubukhulu bephrotheni.
3.Isetshenziswa kumephu ye-peptide
4.Isetshenziselwa ukuqhathanisa ukwakheka kwe-polypeptide yezakhiwo ezahlukene.
5.Isetshenziselwa ukulinganisa ubumsulwa bamaprotheni.
6.Isetshenziswa ku-Western Blotting.
7.Isetshenziswa ekuhloleni i-HIV ukwehlukanisa amaprotheni e-HIV.
8.Ukuhlaziya usayizi kanye nenombolo yama-polypeptide subunits.

Isici

I-DYCZ-24DN yenziwe ngezinto ezibonakalayo zekhwalithi ephezulu, nokubukeka okubucayi, okwamukelwa kabanzi ngamakhasimende ethu. Inezici ezilandelayo:
• Yenziwe ngekhwalithi ephezulu ye-polycarbonate ebonisa ngale, inhle futhi ihlala isikhathi eside, kulula ukuyibuka;
• Ngokufaka ijeli endaweni yokuqala, ekwazi ukuphonsa futhi igijime ijeli endaweni efanayo, kulula futhi kulula ukwenza amajeli, futhi yonge isikhathi sakho esiyigugu;
• Umklamo okhethekile wefreyimu ye-wedge ungalungisa igumbi le-gel ngokuqinile;
• Ithangi lebhafa elibunjiwe elinama-electrode eplatinamu ahlanzekile;
• Kulula futhi kulula ukwengeza amasampula;
• Ukwazi ukusebenzisa ijeli eyodwa noma amabili ngesikhathi esisodwa;
• Gcina isisombululo sebhafa;
• Idizayini ekhethekile yethangi gwema isivimbeli nokuvuza kwejeli;
• Ama-electrode akhiphekayo, kulula ukuwagcina nokuhlanza;
• Cisha ngokuzenzakalelayo lapho isivalo sivuliwe;

I-DYY-6C njengamandla ethu okuthengisa ashisayo ine-voltage ezinzile futhi yamanje. Okulandelayo izici zayo eziyingqayizivele:
• Ukulawula okuhlakaniphile kwephrosesa yekhompyutha;
• Ukwazi ukulungisa amapharamitha ngesikhathi sangempela ngaphansi kwesimo sokusebenza;
• I-LCD yesikrini esikhulu ibonisa i-voltage, yamanje, amandla kanye nesikhathi sesikhathi ngesikhathi esisodwa.
• I-Voltage, yamanje namandla okulawula iluphu evaliwe, ebona ukulungiswa ngesikhathi sokusebenza.
• Ngomsebenzi wokubuyisela.
• Ngemva kokufinyelela isikhathi esimisiwe, inomsebenzi wokugcina wamanje omncane.
• Ukuvikelwa okuphelele kanye nomsebenzi wokuxwayisa kusenesikhathi.
• Ngomsebenzi wokugcina inkumbulo.
• Umshini owodwa onezikhala eziningi, okukhiphayo okune okuhambisanayo.