Iseli ye-Electrophoresis ye-SDS-PAGE kanye ne-Western Blot

Incazelo emfushane:

I-DYCZ-24DN eyeprotheni electrophoresis, kuyilapho i-DYCZ-40D ingeyokudlulisa i-molecule yephrotheni isuka kujeli iye kulwelwesi njengolwelwesi lwe-nitrocellulose ocwaningweni lwe-WesternBlot.Lapha sinenhlanganisela ephelele yamakhasimende ethu engahlangabezana nohlelo lokusebenza ukuthi umhloli angasebenzisa ithangi elilodwa ukuze alenzei-gel electrophoresis, bese ushintshanisa imojula ye-electrode ukwenza isilingo sokucisha ngethangi elifanayo le-DYCZ-24DN.Okudingayo nje isistimu ye-DYCZ-24DN kanye nemojula ye-DYCZ-40D Electrode ezokuvumela ukuthi ushintshe ngokushesha futhi kalula usuke kuhlelo olulodwa lwe-electrophoresis uye kwenye.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ukucaciswa

Ubukhulu (L×W×H)	140×100×150mm
Usayizi weGel (L×W)	75 × 83mm
Ikama	Imithombo eyi-10 nemithombo eyi-15
Ukujiya Kwekama	1.0mm no-1.5mm (Okuvamile) 0.75mm (Uma uthanda)
Inombolo Yamasampuli	20-30
Ivolumu ye-Buffer	400ml
Isisindo	1kg

Incazelo

I-DYCZ-24DN iyiseli eliqondile le-electrophoresis (ithangi/igumbi) le-SDS-PAGE, IKHASI Lomdabu njll. I-protein electrophoresis.Leli seli lingakwazi ukusakaza futhi lisebenzise ijeli endaweni efanayo.Ithambile futhi ikhethekile okulula futhi kulula ukulayisha amasampula.Ithangi lenziwe ngekhwalithi ephezulu ye-polycarbonate, ehlala isikhathi eside futhi esobala.Leli thangi elikhanyayo lenza kube lula ukubona ijeli lapho wenza ucwaningo.I-DYCZ-24DN inama-electrode akhiphekayo okulula ukuwagcina.Ama-Electrodes enziwa ngeplatinamu emsulwa(≥99.95%) eyi-electrolysis-corrosion futhi emelana nezinga lokushisa eliphakeme.

Ngemva kwe-gel electrophoresis, ngokwesidingo sokuhlola, ngezinye izikhathi, umhloli udinga ukudlulisa ijeli ekusekelweni okuqinile ukuze kuhlaziywe okwengeziwe.Kubizwa nge-blotting experiment, okuyindlela yokudlulisa amaprotheni, i-DNA noma i-RNA kusithwali.Kwenziwa ngemva kwe-electrophoresis yejeli, idlulisela ama-molecule asuka kujeli aye kulwelwesi oluvalayo.Ngemva kokucisha, amaprotheni adlulisiwe, i-DNA noma i-RNA abese ebonakala ngokungcoliswa kombala (isibonelo, ukungcoliswa kwesiliva kwamaprotheni), ukubonwa kwe-autoradiographic kwama-molecule ane-radiolabelled (okwenziwa ngaphambi kwe-blot), noma ukulebula okuqondile kwamanye amaprotheni noma ama-nucleic acid.Lokhu kokugcina kwenziwa ngamasosha omzimba noma ama-hybridization probe abopha kuphela ama-molecule athile eblothi futhi abe ne-enzyme ehlanganiswe nawo.Ngemva kokugeza kahle, lo msebenzi we-enzymatic (kanjalo, ama-molecule esiwaseshayo kublothi) ubonakala ngokufukamela ngokusebenza okufanele, okunikeza idiphozithi enombala kublothi noma ukusabela kwe-chemiluminescent okubhaliswe ngefilimu yezithombe.

Ngokunikezwa kwamandla kwaleli seli elime mpo lejeli ye-electrophoresis, sincoma eyodwa ye-timer control electrophoresis amandla imodeli ye-DYY-6C.

Isicelo

Ku-SDS-PAGE, i-electrophoresis ye-Native PAGE nokudlulisa i-molecule yephrotheni isuka kujeli iye kulwelwesi.

Isici

Iseli ye-DYCZ-24DN mini mpo yejeli ye-electrophoresis ye-SDS-PAGE, i-electrophoresis ye-Native PAGE inezici ezilandelayo:

•Yenziwe ngekhwalithi ephezulu ye-polycarbonate esobala, inhle futhi ihlala isikhathi eside, kulula ukuyibuka;

• Ngokusakazwa kwejeli endaweni yokuqala, ekwazi ukuphonsa nokugijima ijeli endaweni efanayo, kulula futhi kulula ukwenza amajeli, futhi yonge isikhathi sakho esiyigugu;

• Umklamo okhethekile wefreyimu ye-wedge ungalungisa igumbi le-gel ngokuqinile;

• Ithangi lebhafa elibunjiwe elinama-electrode eplatinamu ahlanzekile;

• Kulula futhi kulula ukwengeza amasampula;

•Uyakwazi rvula ijeli elilodwa noma amabili ngesikhathi esisodwa;

• Gcina isisombululo sebhafa;

• Idizayini ekhethekile yethangi gwema isivimbeli nokuvuza kwejeli;

•Ama-electrode akhiphekayo, kulula ukuwagcina nokuhlanza;

• Cisha ngokuzenzakalelayo lapho isivalo sivuliwe;

I-electrode module, ebizwa nangokuthi umzimba Osekelayo wokudlulisa noma ukuhlanganiswa kwe-electrode iyingxenye eyinhloko yohlelo lokucisha i-DYCZ-40D.Ihlanganisa izingxenye zombala obomvu nomnyama nama-electrode abomvu namnyama ukuze kuqinisekiswe ukuma okufanele kwejeli ngesikhathi sokudlulisa, kanye nomklamo ophumelelayo owenza kube lula ukufakwa nokukhishwa kwamakhasethi esibambi sejeli eqenjini elisekelayo ukuze lidluliselwe (ukuhlanganiswa kwe-electrode).