Lapha sizochaza indlela yokwenza i-agarose gel electrophoresis ngumcwaningi wethu elebhu ye-Liuyi Biotech.
Ngaphambi kokuhlolwa, sidinga ukuhlola amathuluzi, ama-reagents, nezinye izinto zokuhlola namathuluzi esiwadingayo.
Ukulungiswa kwemishini yokuhlola nezinto zokwakha
Imishini ye-agarose gel electrophoresis
Iseli ye-electrophoresis evundlile (ithangi/igumbi), ukunikezwa kwamandla kagesi we-electrophoresis ophakathi naphansi, isithombe sejeli nohlelo lokuhlaziya.
I-Beijing liuyi Biotechnology Co., Ltd (i-Liuyi Biotech) inikeza amamodeli ahlukene eseli ye-electrophoresis evundlile (ithangi/igumbi) kanye nokuphakelwa kwamandla kanye nesistimu yokubhala imibhalo yejeli.Imodeli yochungechunge lwemikhiqizo ye-DYCP-31 eye-electrophoresis, kanti imikhiqizo yochungechunge lwe-DYY iwumthombo wamandla we-electrophoresis.Imikhiqizo yochungechunge lwe-WD-9413 iwuhlelo lokubhalwa kwejeli.Kusukela kusayizi omncane wejeli 60×60mm kuya kujeli omkhulu ongu-250×250mm, singahlangabezana nezidingo zakho ezihlukene zosayizi wejeli.ImodeliI-DYCP-32Cingenza ijeli ifinyelele usayizi 250×250mm.II-DYY-6Cugesi wethu.Isekela okukhiphayo okungu-400V, 400mA, 240W, okuwumkhiqizo wethu ovamile osetshenziswa amakhasimende ethu. I-WD-9413BI-Gel Image & Analysis System isetshenziselwa ukuhlaziya nokucwaninga ijeli, amafilimu, nama-blots ngemva kokuhlolwa kwe-electrophoresis.Kuyidivayisi eyisisekelo enomthombo wokukhanya kwe-ultraviolet yokubuka nokuthwebula amajeli angcoliswe odayi be-fluorescent njenge-ethidium bromide, kanye nomthombo wokukhanya omhlophe wokubuka nokuthwebula amajeli angcoliswe odayi abafana nohlaza okwesibhakabhaka okucwebezelayo.
Ama-reagents we-agarose gel electrophoresis
1.I-medium: ijeli ye-agarose
2.Isigcinalwazi: TAE (Tris-acetate, EDTA, glacial acetic acid) kanye ne-TBE ( Tris-borate, EDTA, boric acid ).
3.Isigcinalwazi sokulayisha: 6×DNA Loading Buffer (Special for DNA sample: EDAT, glycerine,Xylene cyanol and bromophenol blue)
Udayi
Udayi we-fluorescent njengo-EB, Gelred, goldview, GenGreen, GenView, SYBRGreen
IIzinto ezisetshenziswayo
Amathiphu epayipi lokuvala inzalo (10μL), amathiphu epayipi (200μL), amathiphu epayipi (1000μL), 200μL\500μL\1.5ml centrifuge tube.
I-DNA Marker
Ilungiselelwe ngokwesisindo samangqamuzana okufanele ihlolwe.
I-Gel igijima futhi ibuke
Okokuqala, sidinga ukulungisa ijeli ye-agarose.Ukugcwala kwe-agarose kujeli kuzoncika kumasayizi wezingcezu ze-DNA ezizohlukaniswa, namajeli amaningi asukela phakathi kuka-0.5% -2%.Ukuthatha wethuI-DYCP-31DNisibonelo, Ungaphonsa ijeli encane, ijeli ebanzi, ijeli ende kanye nejeli yesikwele ngokwezidingo zokuhlolwa.Khetha ithreyi yejeli oyidingayo, bese ufaka ikama, bese uthela ijeli ye-agarose eshisayo emshinini wokuphonsa wejeli.
Ngemva kwalokho, ngemva kokuba ijeli seliqinile, susa ikamu ngokucophelela nangokuthambile, bese ufaka ithreyi yejeli ethangini le-buffer.Thela isisombululo se-buffer ethangini le-buffer futhi wenze yonke ijeli icwilise ku-buffer.Layisha amasampula emithonjeni nge-pipette ejwayelekile.Xhuma i-I-DYCP-31DNnge-electrophoresis power supply ngendlela efanele, bese usetha imingcele ukuze usebenzise ijeli.
Bona ngeso lengqondo izingcezwana zakho ze-DNA ku-transilluminator ye-UV.
Angemuva kokusebenzisa ijeli, ungasebenzisa isithombe sethu sejeli nemodeli yesistimu yokuhlaziyaI-WD-9413Bukubuka, ukuhlaziya nokuthatha izithombe zejeli.I-Liuyi Biotech futhi inikeza i-UV transilluminator (UV Analyzer) ukuze ibone ijeli.Sinebhokisi elimnyama uhlobo lwe-UV trimodeli ye-ansilluminator (UV Analyzer).I-WD-9403A, 9403C, I-WD-9403F, imodeli ephathekayo ye-UV transilluminator (UV Analyzer).I-WD-9403Bkanye ne-handhold UV transilluminator (UV Analyzer)I-WD-9403Engoba ukhetha.
Isithombe se-gel post electrophoresis
I-Liuyi brand inomlando weminyaka engaphezu kwengama-50 e-China futhi inkampani ingahlinzeka ngemikhiqizo ezinzile nesezingeni eliphezulu emhlabeni jikelele.Ngeminyaka yokuthuthuka, kufanelekile ukukhetha kwakho!
Ukuze uthole ukwaziswa okwengeziwe ngathi, sicela usithinte nge-imeyili[i-imeyili ivikelwe], [i-imeyili ivikelwe].
Isikhathi sokuthumela: Apr-29-2022