Agarosegel ei-lectrophoresis
I-Agarose gel electrophoresis iyindlela ye-gel electrophoresis esetshenziswa ku-biochemistry, biology yamangqamuzana, izakhi zofuzo, kanye namakhemikhali emtholampilo ukuze kuhlukaniswe inani elixubile lama-macromolecules njenge-DNA nomaI-RNA.Lokhu kufezwa ngokuhambisa ama-nucleic acid ama-nucleic acid ashajwa kabi nge-agarose matrix ene-agarose matrix.i-electrophoresis.
Izici zejeli le-agarose
Ijeli ye-Agarose iyi-matrix enezinhlangothi ezintathu ezakhiwe ngamamolekyuli e-agarose e-helical ezigoqweni ezinkulu ezihlanganiswe zibe izakhiwo ezinezinhlangothi ezintathu ezinemigudu nezimbotshana lapho ama-biomolecules angadlula khona. ngokufudumeza kubuyele esimweni esiwuketshezi.Izinga lokushisa elincibilikayo lihlukile ekushiseni kwe-gelling, kuye ngemithombo, ijeli ye-agarose inokushisa kwe-gelling engu-35.-42 °C kanye nezinga lokushisa elincibilikayo elingu-85-95 °C.I-agaroses encibilika kancane kanye ne-low-gelling enziwe ngokuguqulwa kwamakhemikhali ayatholakala.
Izinzuzo kanye nokubi kwe-agarose gel electrophoresis
Inzuzo eyinhloko yenqubo ye-agarose gel electrophoresis ukuthi ingacutshungulwa kalula futhi i-molecule ye-DNA esetshenziswa njengesampula nayo ingatholwa ngaphandle kokulimala kuyo ekupheleni kwenqubo.Ngoba ijeli ye-agarose ayifuni't iqukethe i-sulfate neqembu le-carboxyl, ngeke ibangele i-electroosmosis ye-agar.Ngesakhiwo esifanayo kanye nosayizi omkhulu wembotshana, ijeli ye-agarose ilungeleukuhlukanisa ama-molecule amakhulu e-DNA, njenge i-enzyme eyinkimbinkimbi, i-nucleic acid kanye negciwane.Ijeli ye-Agarose ayifuni'imunca ukukhanya kwe-UV, ngakho-ke ingaba namabala ngokuqondile noma ngemva kokugaywa ukuze ibe ifilimu, iyakwazi ukubuyiselwa emuva ngokushisa.
Kukhona futhi ukungalungi kwenqubo ye-agarose gel electrophoresis.Ijeli ye-Agarose iphuka kalula, ngakho-ke ukuminyana akukwazi ukuba ngaphansi kakhulu, kuzobiza i-agarose eyengeziwe.Ijeli ye-agarose kunzima ukuyigcina ngoba kulula ukungcoliswa amagciwane, futhi kufanele ilungiswe ngaphambi nje kokuyisebenzisa.Uma kuqhathaniswa ne-PAGE electrophoresis technique, ukusebenza kahle kwe-molecular sieve kuncane, futhi indawo ye-electrophoresis incane.
Okuvundlileei-lectrophoresisuhlelo lwejeli ye-agarose
Ngaphandle kwe-agarose ne-buffer, ukuhlolwa kwe-agarose gel electrophoresis kudinga uhlelo lwe-electrophoresis oluvundlile kanye ne-low-Ukunikezwa kwamandla kwe-voltage electrophoresis.I-Beijing Liuyi Biotechnology inganikeza uhlelo lwe-electrophoresis oluvundlile futhi Ukunikezwa kwamandla kwe-electrophoresis, njengemodeliI-DYCP-31BN, I-DYCP-31CN okweisistimu ye-electrophoresis enezingqimba futhiI-DYY-6C kokunikezwa kwamandla.
Kuya kumasayizi ahlukene ejeliedingwa yi- ukuhlolwa kwe-agrose gel electrophoresis, osayizi abahlukene besistimu ye-electrophoresis evundlile inganikezwa yi-Beijing Liuyi Biotechnology.Imodeli yochungechunge lwe-DYCP-31,sukh njengobaI-DYCP-31BN, I-DYCP-31CN, I-DYCP-31DN, I-DYCP-31E, I-DYCP-32B, futhiI-DYCP-32Cbanosayizi abahlukene bethreyi yejeli, kusukela ekusakazeni usayizi omncane wejeli ukuya kujeli kasayizi omkhulu, bangahlangabezana nokuhlola kwakhoizidingo.
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Ukuze uthole ukwaziswa okwengeziwe ngathi, sicela usithinte nge-imeyili[i-imeyili ivikelwe], ukuthengisa01@ly.com.cn.
Isikhathi sokuthumela: Apr-16-2022