I-DNA Gel Electrophoresis: Ukuhlaziya Izingcezu Zofuzo

I-DNA gel electrophoresis iyindlela evamile yebhayoloji yamangqamuzana esetshenziselwa ukuhlukanisa nokuhlaziya izingcezu ze-DNA ngokusekelwe kosayizi bazo.Le nqubo ihilela ukulayisha izingcezu ze-DNA ezinosayizi obuhlukene ejeli eyenziwe nge-agarose, icarbohydrate etholakala kulwelwe olubomvu.

Ukulungiselela nokuphonsa ijeli ye-agarose

1. Chaza i-agarose ngenani elifanele le-electrophoresis buffer.Ukugxiliswa kwejeli kunqunywa isilinganiso sesisindo-to-volume, njenge-1g ye-agarose ku-100.ml yebuffer yejeli engu-1%.
2. Shisisa ingxube ku-microwave, uzungezisa isitsha ukuze uqinisekise ukuqedwa okuphelele kwe-agarose.
3. Engeza i-ethidium bromide kusixazululo sejeli ekuhlanganiseni kokugcina kwe-0.5mg/ ml.I-Ethidium bromide ihlangana phakathi kwamapheya esisekelo e-DNA aseduze futhi ikhipha i-fluorescence ewolintshi ngaphansi kokukhanya kwe-UV.Qaphela ukuthi i-ethidium bromide iyi-carcinogen, ngakho ukuyiphatha kudinga izinyathelo ezifanele zokuphepha njengokugqoka amagilavu.
4. Pholisa isisombululo sejeli emanzini okugeza ukuvimbela ithreyi yejeli ukuthi ingaguquki ngenxa yamazinga okushisa aphezulu.
5. Faka ikamu ekhambi lejeli ukuze wenze isampula yemithombo.Khetha amakamu afanele inani lesampula le-DNA ozolilayisha.Thela isisombululo sejeli ye-agarose kuhhaviniithreyi yejelifuthi uyivumele ukuthi iqine ekamelweni lokushisa.
6. Uma ijeli seliqinile, susa ikamu.Uma ungeke usebenzise ijeli ngokushesha, yibophe ngepulasitiki bese uyigcina ku-4℃ kuze kube yilapho idingeka.

Ukulungiselela nokusebenzisa ijeli

Ngaphambi kokuqala i-electrophoresis, hlanganisa isampula ye-DNA nebhafa yokulayisha.Ibhafa yokulayisha ivamise ukugxila ngokuphindwe kasithupha futhi isiza isampula lizike phansi kwemithombo futhi lilandelele ukunyakaza ngesikhathi se-electrophoresis.

Setha ukunikezwa kwamandla ku-voltage ecacisiwe.

Engeza i-electrophoresis buffer eyanele ethangini lejeli ukumboza indawo yejeli.Qinisekisaizixhumanisi ezifanele zama-electrode.

Layisha isampula ye-DNA kanye nezimpawu zesisindo samangqamuzana emithonjeni yejeli.

Vula ukunikezwa kwamandla ukuze uqalise i-electrophoresis.

Ukubheka izingcezu ze-DNA ezihlukanisiwe

Ngemuva kwe-electrophoresis, cisha ukunikezwa kwamandla bese ususa ijeli.

Sebenzisa umthombo wokukhanya we-UV ukuze ukhanyise ijeli;Izingcezu ze-DNA zizovela njengamabhande awolintshi e-fluorescent.

Bhala isithombe sejeli ukuze uhlaziye izingcezu ze-DNA ezihlukanisiwe.

Ngemuva kokuhlolwa, qinisekisa ukulahlwa okufanele kwejeli ne-electrophoresis buffer, ulandela imihlahlandlela yaselabhorethri.Gqoka amagilavu ​​ngaso sonke isikhathi lapho uphatha amajeli namabhafa aqukethe i-ethidium bromide ukuze uvikele ekuchayekeni kule nto eyingozi.

Ijeli ye-DNA electrophoresis isetshenziswa kakhulu kubhayoloji yamangqamuzana nocwaningo lwezakhi zofuzo ukuze kulinganiswe osayizi be-molecule ye-DNA, ukuhlukanisa izingcezu ze-DNA, ukuthola ukuguqulwa kofuzo, nokunyatheliswa kweminwe kwe-DNA, phakathi kwezinye izinhlelo zokusebenza.Kuyindlela elula nephumelelayo yokuhlola esiza ekuqondeni ukwakheka nezici zamasampula e-DNA.

I-Beijing Liuyi Biotechnology Co.Ltd, umkhiqizi ochwepheshe ogxile emikhiqizweni ye-electrophoresis ngaphezu kweminyaka engama-50, unikeza amathangi e-electrophoresis (amakamelo/amaseli) e-gel electrophoresis, njengamathangi e-electrophoresis avundlile (amakamelo/amaseli)I-DNAi-electrophoresis yejeli, namathangi e-electrophoresis aqondile (amagumbi/amaseli) we-protein gel electrophoresis.Phakathi naleso sikhathi, ihlinzeka ngezinhlobo ezahlukene zamandla kagesi we-electrophoresis, i-cabinet dark box analyzer kanye ne-UV transilluminator.Ungakhetha amathangi e-electrophoresis ka-Beijing Liuyi (amagumbi/amaseli) ukuze ulinganise isisindo samangqamuzana e-RNA futhi uhlukanise i-RNA yosayizi abahlukahlukene ngelebhu yakho.

Lapha sizokwenzancomauhlobo olulodwa lwe-electrophoresis tank evundlile (igumbi/iseli) imodeliI-DYCP-31DN ukwenza nokusebenzisa ijeli.

Ngemuva kokusebenzisa ijeli, ungasebenzisa isithombe sethu sejeli nemodeli yesistimu yokuhlaziyaI-WD-9413Bukubuka, ukuhlaziya nokuthatha izithombe zejeli.I-Liuyi Biotech futhi inikeza i-UV transilluminator (UV Analyzer) ukuze ibone ijeli.Sinemodeli yebhokisi elimnyama lohlobo lwe-UV transilluminator (UV Analyzer).I-WD-9403A,9403C,I-WD-9403F, imodeli ephathekayo ye-UV transilluminator (UV Analyzer).I-WD-9403Bkanye ne-handhold UV transilluminator (UV Analyzer)I-WD-9403Engoba ukhetha.

I-Beijing Liuyi brand inomlando weminyaka engaphezu kwengama-50 e-China futhi inkampani ingahlinzeka ngemikhiqizo ezinzile nesezingeni eliphezulu emhlabeni jikelele.Ngeminyaka yokuthuthuka, kufanelekile ukukhetha kwakho!

Manje sifuna ozakwethu, kokubili ithangi le-OEM electrophoresis kanye nabasabalalisi bamukelekile.

Uma unecebo lokuthenga lemikhiqizo yethu, sicela ungangabazi ukusithinta.Ungasithumela umlayezo nge-imeyili[i-imeyili ivikelwe]noma[i-imeyili ivikelwe], noma sicela usishayele ku- +86 15810650221 noma wengeze uWhatsapp +86 15810650221, noma Wechat: 15810650221.