I-DNA Gel Electrophoresis: Ukuhlaziya Izingcezu Zofuzo

I-DNA gel electrophoresis iyindlela evamile yebhayoloji yamangqamuzana esetshenziselwa ukuhlukanisa nokuhlaziya izingcezu ze-DNA ngokusekelwe kosayizi bazo. Le nqubo ihilela ukulayisha izingcezu ze-DNA ezinosayizi obuhlukile ejeli eyenziwe nge-agarose, icarbohydrate etholakala kulwelwe olubomvu.

Ukulungiselela nokuphonsa ijeli ye-agarose

1. Chaza i-agarose ngenani elifanele le-electrophoresis buffer. Ukugxila kwejeli kunqunywa isilinganiso sesisindo-to-volume, njenge-1g ye-agarose ku-100.ml yebuffer yejeli engu-1%.
2. Shisisa ingxube ku-microwave, uzungezisa isitsha ukuze uqinisekise ukuqedwa okuphelele kwe-agarose.
3. Engeza i-ethidium bromide kusixazululo sejeli ekuhlanganiseni kokugcina kwe-0.5mg/ ml. I-Ethidium bromide ihlangana phakathi kwamapheya esisekelo e-DNA aseduze futhi ikhipha i-fluorescence ewolintshi ngaphansi kokukhanya kwe-UV. Qaphela ukuthi i-ethidium bromide iyi-carcinogen, ngakho ukuyiphatha kudinga izinyathelo ezifanele zokuphepha njengokugqoka amagilavu.
4. Pholisa isisombululo sejeli emanzini okugeza ukuze uvimbele i-tray yejeli ukuthi ingaguquki ngenxa yamazinga okushisa aphezulu.
5. Faka ikamu ekhambi lejeli ukuze wenze isampula yemithombo. Khetha amakamu afanele inani lesampula le-DNA ozolilayisha. Thela isisombululo sejeli ye-agarose kuhhaviniithreyi yejelifuthi uyivumele ukuthi iqine ekamelweni lokushisa.
6. Uma ijeli seliqinile, susa ikamu. Uma ungeke usebenzise ijeli ngokushesha, yibophe ngepulasitiki bese uyigcina ku-4℃ kuze kube yilapho idingeka.

Ukulungiselela nokusebenzisa ijeli

Ngaphambi kokuqala i-electrophoresis, hlanganisa isampula ye-DNA nebhafa yokulayisha. Ibhafa yokulayisha ivamise ukugxila ngokuphindwe kasithupha futhi isiza isampula lizike phansi kwemithombo futhi lilandelele ukunyakaza ngesikhathi se-electrophoresis.

Setha ukunikezwa kwamandla ku-voltage ecacisiwe.

Engeza i-electrophoresis buffer eyanele ethangini lejeli ukumboza indawo yejeli.Qinisekisaizixhumanisi ezifanele zama-electrode.

Layisha isampula ye-DNA kanye nezimpawu zesisindo samangqamuzana emithonjeni yejeli.

Vula ukunikezwa kwamandla ukuze uqalise i-electrophoresis.

Ukubheka izingcezu ze-DNA ezihlukanisiwe

Ngemuva kwe-electrophoresis, cisha ukunikezwa kwamandla bese ususa ijeli.

Sebenzisa umthombo wokukhanya we-UV ukuze ukhanyise ijeli; Izingcezu ze-DNA zizovela njengamabhande awolintshi e-fluorescent.

Bhala isithombe sejeli ukuze uhlaziye izingcezu ze-DNA ezihlukanisiwe.

Ngemuva kokuhlolwa, qinisekisa ukulahlwa okufanele kwejeli ne-electrophoresis buffer, ulandela imihlahlandlela yaselabhorethri. Gqoka amagilavu ​​ngaso sonke isikhathi lapho uphatha amajeli namabhafa aqukethe i-ethidium bromide ukuze uvikele ekuchayekeni kule nto eyingozi.

I-DNA gel electrophoresis isetshenziswa kakhulu kubhayoloji yamangqamuzana nocwaningo lwezakhi zofuzo ukulinganisa osayizi be-molecule ye-DNA, ukuhlukanisa izingcezwana ze-DNA, ukuthola ukuguqulwa kofuzo, nokunyatheliswa kweminwe kwe-DNA, phakathi kwezinye izinhlelo zokusebenza. Kuyindlela elula nephumelelayo yokuhlola esiza ekuqondeni ukwakheka nezici zamasampula e-DNA.

I-Beijing Liuyi Biotechnology Co.Ltd, umkhiqizi ochwepheshe ogxile emikhiqizweni ye-electrophoresis ngaphezu kweminyaka engama-50, unikeza amathangi e-electrophoresis (amakamelo/amaseli) e-gel electrophoresis, njengamathangi e-electrophoresis avundlile (amagumbi/amaseli)I-DNAi-electrophoresis yejeli, namathangi e-electrophoresis aqondile (amagumbi/amaseli) we-protein gel electrophoresis. Phakathi naleso sikhathi, ihlinzeka ngezinhlobo ezahlukene zokunikezwa kwamandla kagesi we-electrophoresis, i-cabinet dark box analyzer kanye ne-UV transilluminator. Ungakhetha amathangi e-electrophoresis ka-Beijing Liuyi (amagumbi/amaseli) ukuze ulinganise isisindo samangqamuzana e-RNA futhi uhlukanise i-RNA yosayizi abahlukahlukene ngelebhu yakho.

Lapha sizokwenzancomauhlobo olulodwa lwe-electrophoresis tank evundlile (igumbi/iseli) imodeliI-DYCP-31DN ukwenza nokusebenzisa ijeli.

Ngemuva kokusebenzisa ijeli, ungasebenzisa isithombe sethu sejeli nemodeli yesistimu yokuhlaziyaI-WD-9413Bukubuka, ukuhlaziya nokuthatha izithombe zejeli. I-Liuyi Biotech futhi inikeza i-UV transilluminator (UV Analyzer) ukuze ibone ijeli. Sinemodeli yebhokisi elimnyama lohlobo lwe-UV transilluminator (UV Analyzer).I-WD-9403A,9403C,I-WD-9403F, imodeli ephathekayo ye-UV transilluminator (UV Analyzer).I-WD-9403Bkanye ne-handhold UV transilluminator (UV Analyzer)I-WD-9403Engoba ukhetha.

I-Beijing Liuyi brand inomlando weminyaka engaphezu kwengama-50 e-China futhi inkampani ingahlinzeka ngemikhiqizo ezinzile nesezingeni eliphezulu emhlabeni jikelele. Ngeminyaka yokuthuthuka, kufanelekile ukukhetha kwakho!

Manje sifuna ozakwethu, kokubili ithangi le-OEM electrophoresis kanye nabasabalalisi bamukelekile.

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