I-Gel electrophoresis ingenye yezindlela ezinkulu ezisetshenziswa kubhayoloji yamangqamuzana ekuhlaziyeni i-DNA.Le ndlela ihilela ukufuduka kwezingcezu ze-DNA ngokusebenzisa ijeli, lapho zihlukaniswa khona ngokusekelwe ngobukhulu noma ukuma.Kodwa-ke, ingabe uke wabhekana namaphutha phakathi nokuhlolwa kwe-electrophoresis, njengamabhande agcotshwe kujeli ye-agarose, noma awekho amabhande ejeli?Kungaba yini imbangela yala maphutha?
Ochwepheshe bethu bafingqa imibhangqwana yokuxazulula inkinga lapha ukuze uthole ireferensi yakho.
1. Amabhande agcotshwe kujeli ye-agarose
●I-DNA yehlisiwe.Gwema ukungcoliswa kwe-nuclease.
● Ibhafa ye-electrophoresis ayisintsha.Ngemuva kokusetshenziswa okuphindaphindiwe kwe-electrophoresis buffer, amandla e-ionic ayancipha, futhi inani layo le-pH liyakhuphuka, ngakho umthamo we-buffer uba buthaka, okuthinta umphumela we-electrophoresis.Kunconywa ukufaka esikhundleni se-electrophoresis buffer njalo.
● Kusetshenziswe izimo ezingafanele ze-electrophoresis.Ungavumeli i-voltage yeqe ku-20 V/cm, futhi ugcine izinga lokushisa elingu-<30° C ngesikhathi se-electrophoresis.Nge-electrophoresis ye-DNA strand enkulu, izinga lokushisa kufanele libe ngaphansi kuka-15° C. Hlola ukuthi isigcinalwazi se-electrophoresis sinomthamo webhafa owanele.
● Kufakwe i-DNA eningi kujeli.Yehlisa inani le-DNA.
● Usawoti omningi kuyi-DNA.Sebenzisa i-ethanol precipitation ukuze ususe usawoti oweqile uma usuthuthukile.
● I-DNA yayingcoliswe amaprotheni.Sebenzisa i-phenol extractions ukuze ususe amaprotheni asethuthukile.
● I-DNA yashintshwa.Ungashisi ngaphambi kwe-electrophoresis.Nciphisa i-DNA ku-buffer nge-20 mM NaCl.
2. Okudidayo ukufuduka kwebhendi ye-DNA
● Ukwenziwa kabusha kwesayithi ye-COS yesiqephu esingu-λHind III.Shisisa i-DNA imizuzu emi-5 ngaphansi kuka-65° C ngaphambi kwe-electrophoresis, bese uyipholisa kuyunithi yeqhwa imizuzu emi-5.
● Kusetshenziswe izimo ezingafanele ze-electrophoresis.Ungavumeli i-voltage yeqe ku-20 V/cm, futhi ugcine izinga lokushisa elingu-<30° C ngesikhathi se-electrophoresis.Hlola ukuthi i-electrophoresis buffer inomthamo owanele webhafa.
● I-DNA yashintshwa.Ungashisi ngaphambi kwe-electrophoresis.Nciphisa i-DNA ku-buffer nge-20 mM NaCl.
3. Amabhande e-DNA aqulekile noma awekho kujeli ye-agarose
● Kube nenani elinganele noma ukugcwala kwe-DNA elayishwe kujeli.Khulisa inani le-DNA.I-Polyacrylamide gel electrophoresis izwela kancane kune-agarose electrophoresis, futhi ukulayishwa kwesampula kungehliswa ngokufanelekile.
● I-DNA yonakaliswa.Gwema ukungcoliswa kwe-nuclease.
● I-DNA yafakwa i-electrophored kujeli.I-Electrophorese ijeli isikhathi esincane, sebenzisa i-voltage ephansi, noma sebenzisa ijeli ephakeme kakhulu.
● Umthombo wokukhanya oku-W ongalungile usetshenziswe ukuze kubonakale ngeso lengqondo i-DNA enebala le-ethidium bromide.Sebenzisa ukukhanya kwe-shortwavelength (254 nm) W ukuze uthole ukuzwela okukhulu.
4. Amabhande e-DNA alahlekile
●I-DNA yosayizi omncane ikhishwe nge-electrophored kujeli.I-Electrophorese ijeli isikhathi esincane, sebenzisa i-voltage ephansi, noma sebenzisa ijeli ephakeme kakhulu.
● Kunzima ukuhlukanisa amaqoqo e-DNA amangqamuzana afanayo.Khulisa isikhathi se-electrophoresis, futhi uhlole ukuhlushwawejeli ukuze uqiniseke ukuthi ijeli isetshenziswe ngendlela efanele.
● I-DNA yashintshwa.Ungashisi ngaphambi kwe-electrophoresis.Nciphisa i-DNA ku-buffer nge-20 mM NaCl.
● Izintambo ze-DNA zinkulu, futhi i-electrophoresis yejeli evamile ayifaneleki.Hlaziya nge-pulse gel electrophoresis.Yiziphi ezinye izinkinga oye waba nazo nge-agarose gel electrophoresis?Sizocwaninga kabanzi ukuthola imihlahlandlela esikhathini esizayo.
I-Beijing Liuyi biotechnology Co., Ltd (i-Liuyi Biotech) yinkampani ekhethekile egxile emikhiqizweni ehlobene ne-electrophoresis e-China.Indaba yayo iqala ngo-1970 lapho iChina yayingakangeni ekuguquleni nasekuvuleni isikhathi.Ngokuthuthuka kweminyaka, i-Liuyi Bitotech inomkhiqizo wayo, owaziwa ngokuthi i-Liuyi Brand emakethe yasekhaya yemikhiqizo ye-electrophoresis.
Uhlobo lwe-Liuyi lunomlando ongaphezu kweminyaka engama-50 e-China futhi inkampani ingahlinzeka ngemikhiqizo ezinzile nesezingeni eliphezulu emhlabeni jikelele.Ngokuthuthuka kweminyaka, kufanelekile ukukhetha kwakho!
Amaseli e-electrophoresis avundlile (amathangi/amagumbi) e-Liuyi Biotech ayikhwalithi ephezulu futhi abukeka kahle.Ngosayizi abahlukahlukene bamathreyi ejeli, angahlangabezana nezidingo zakho ezihlukene zokuhlola.Sinethimba lethu lobuchwepheshe kanye nefekthri.Kusukela ekuklanyweni kuya ekukhiqizeni, izinto zokusetshenziswa kuya ezingxenyeni ezibalulekile, singakwazi ukulawula yonke inqubo.Uchungechunge lwe-DYCP 31 olwe-DNA electrophoresis, okuyimodeliI-DYCP-31BN, I-DYCP-31CN,I-DYCP-31DN, futhiI-DYCP-31E.Umehluko phakathi kwabo ubukhulu bejeli kanye nentengo.Sihlinzeka ngosayizi abagcwele bemikhiqizo kumakhasimende ethu.ImodeliI-DYCP-32Cingenza ijeli enkulu ibe ngu-250mm*250mm.
Okwamanje, sincoma ukunikezwa kwamandla kwe-electrophoresis yethuI-DYY-6C,I-DYY-6DfuthiI-DYY-10Ckumaseli ethu e-electrophoresis (amathangi/amagumbi) i-DYCP-31 kanye nochungechunge lwama-32.
Uma ufuna ulwazi olwengeziwe mayelana nemikhiqizo, sicela uvakashele le webhusayithi ukuze uthole okwengeziwe, futhi wamukelekile ukusithinta nge-imeyili ukuze usazise ukuthi yini oyifunayo, futhi ubone ukuthi singakunikeza yini izixazululo.
Ukuze uthole ukwaziswa okwengeziwe ngathi, sicela usithinte nge-imeyili[i-imeyili ivikelwe], [i-imeyili ivikelwe].
Isikhathi sokuthumela: May-09-2022