Iseli ye-Electrophoresis ye-SDS-PAGE kanye ne-Western Blot
Ukucaciswa
| Ubukhulu (L×W×H) | 140×100×150mm |
| Usayizi weGel (L×W) | 75 × 83mm |
| Ikama | Imithombo eyi-10 nemithombo eyi-15 |
| Ukujiya Kwekama | 1.0mm no-1.5mm (Okuvamile) 0.75mm (Uma uthanda) |
| Inombolo Yamasampuli | 20-30 |
| Ivolumu ye-Buffer | 400ml |
| Isisindo | 1kg |
Incazelo
I-DYCZ-24DN iyiseli eliqondile le-electrophoresis (ithangi/igumbi) le-SDS-PAGE, IKHASI Lomdabu njll. I-protein electrophoresis. Leli seli lingakwazi ukusakaza futhi lisebenzise ijeli endaweni efanayo. Ithambile futhi ikhethekile okulula futhi kulula ukulayisha amasampula. Ithangi lenziwe ngekhwalithi ephezulu ye-polycarbonate, ehlala isikhathi eside futhi esobala. Leli thangi elikhanyayo lenza kube lula ukubona ijeli lapho wenza ucwaningo. I-DYCZ-24DN inama-electrode akhiphekayo okulula ukuwagcina. Ama-Electrodes enziwa ngeplatinamu emsulwa(≥99.95%) eyi-electrolysis-corrosion futhi emelana nezinga lokushisa eliphakeme.
Ngemuva kwe-gel electrophoresis, ngokwesidingo sokuhlola, ngezinye izikhathi, umhloli udinga ukudlulisa ijeli ekusekelweni okuqinile ukuze kuhlaziywe okwengeziwe. Kubizwa nge-blotting experiment, okuyindlela yokudlulisa amaprotheni, i-DNA noma i-RNA kusithwali. Kwenziwa ngemva kwe-electrophoresis yejeli, idlulisela ama-molecule asuka kujeli aye kulwelwesi oluvalayo. Ngemva kokucisha, amaprotheni adlulisiwe, i-DNA noma i-RNA abe esebonakala ngokungcoliswa kombala (isibonelo, ukungcoliswa kwesiliva kwamaprotheni), ukubonwa kwe-autoradiographic kwama-molecule ane-radiolabelled (okwenziwa ngaphambi kwe-blot), noma ukulebula okuqondile kwamanye amaprotheni noma ama-nucleic acid. Lokhu kokugcina kwenziwa ngamasosha omzimba noma ama-hybridization probe abopha kuphela ama-molecule athile eblothi futhi ane-enzyme ehlanganiswe nawo. Ngemva kokugeza kahle, lo msebenzi we-enzymatic (kanjalo, ama-molecule esiwaseshayo kublothi) ubonakala ngokufukamela ngokusebenza okufanele, okunikeza idiphozithi enombala kublothi noma ukusabela kwe-chemiluminescent okubhaliswe ngefilimu yezithombe.
Ngokunikezwa kwamandla kwaleli seli elime mpo lejeli ye-electrophoresis, sincoma eyodwa ye-timer control electrophoresis amandla imodeli ye-DYY-6C.
Isicelo
Ku-SDS-PAGE, i-electrophoresis ye-Native PAGE nokudlulisa i-molecule yephrotheni isuka kujeli iye kulwelwesi.
Isici
Iseli ye-DYCZ-24DN mini mpo yejeli ye-electrophoresis ye-SDS-PAGE, i-electrophoresis ye-Native PAGE inezici ezilandelayo:
•Yenziwe ngekhwalithi ephezulu ye-polycarbonate esobala, inhle futhi ihlala isikhathi eside, kulula ukuyibuka;
• Ngokufaka ijeli endaweni yokuqala, ekwazi ukuphonsa futhi igijime ijeli endaweni efanayo, kulula futhi kulula ukwenza amajeli, futhi yonge isikhathi sakho esiyigugu;
• Umklamo okhethekile wefreyimu ye-wedge ungalungisa igumbi le-gel ngokuqinile;
• Ithangi lebhafa elibunjiwe elinama-electrode eplatinamu ahlanzekile;
• Kulula futhi kulula ukwengeza amasampula;
•Uyakwazi rvula ijeli elilodwa noma amabili ngesikhathi esisodwa;
• Gcina isisombululo sebhafa;
• Idizayini ekhethekile yethangi gwema isivimbeli nokuvuza kwejeli;
•Ama-electrode akhiphekayo, kulula ukuwagcina nokuhlanza;
• Cisha ngokuzenzakalelayo lapho isivalo sivuliwe;
I-electrode module, ebizwa nangokuthi umzimba Osekelayo wokudlulisa noma ukuhlanganiswa kwe-electrode iyingxenye eyinhloko yohlelo lokucisha i-DYCZ-40D. Ihlanganisa izingxenye zombala obomvu nomnyama nama-electrode abomvu namnyama ukuze kuqinisekiswe ukuma okufanele kwejeli ngesikhathi sokudlulisa, kanye nomklamo ophumelelayo owenza kube lula ukufakwa nokukhishwa kwamakhasethi esibambi sejeli endikimbeni esekelayo ukuze idluliselwe (ukuhlanganiswa kwe-electrode).
