I-Pulsed Field Gel Electrophoresis CHEF Mapper A1
Imininingwane
| Imodeli | I-CHEF Mapper A1 |
| I-Voltage Gradient | 0.5V/cm ukuya ku-9.6V/cm, inyuswe ngo-0.1V/cm |
| Ubuningi Bamanje | 0.5A |
| Amandla kagesi aphezulu | 350V |
| I-Pulse Angle | ±120° |
| I-Time Gradient | Umugqa |
| Ukushintsha Isikhathi | 50ms kuya ku-18h |
| Isikhathi esiphezulu sokugijima | 999h |
| Inombolo yama-Electrodes | 24, ilawulwa ngokuzimela |
| Izinga Lokushisa | 0℃ kuya ku-50℃, iphutha lokubona <±0.5℃ |
Incazelo
I-pulsed field gel electrophoresis (PFGE) ihlukanisa ama-molecule e-DNA ngokushintshanisa inkambu kagesi phakathi kwamapheya ama-electrode agxile endaweni ahlukene, okubangele ama-molecule e-DNA, angaba izigidi zamapheya ayisisekelo ubude ukuze ahlele kabusha futhi afuduke ngezimbotshana zejeli ye-agarose ngesivinini esihlukile. Ifinyelela ukulungiswa okuphezulu ngaphakathi kwalolu hlu futhi isetshenziswa kakhulu kubhayoloji yokwenziwa; ukuhlonza imigqa yezinto eziphilayo kanye ne-microbial; ucwaningo ku-epidemiology yamangqamuzana; izifundo zezingcezu ezinkulu ze-plasmid; ukwenziwa kwasendaweni kwezakhi zofuzo zezifo; imephu ebonakalayo yezakhi zofuzo, ukuhlaziywa kwe-RFLP, nokunyathelisa iminwe ye-DNA; ucwaningo lokufa kwamangqamuzana oluhleliwe; izifundo ngokulimala nokulungiswa kwe-DNA; ukuhlukaniswa nokuhlaziywa kwe-DNA ye-genomic; ukuhlukaniswa kwe-chromosomal DNA; ukwakhiwa, ukuhlonza, kanye nokuhlaziywa kwemitapo yolwazi eneziqephu ezinkulu; kanye nokugxila kwe-transgenic research.t okuphansi njengokungu-0.5 ng/µL (dsDNA).
Isicelo
Ifanele ukuhlonza nokuhlukanisa ama-molecule e-DNA asuka ku-100bp kuya ku-10Mb ngosayizi, okuthola ukulungiswa okuphezulu ngaphakathi kwalolu banga.
Isici
• Ubuchwepheshe Obuthuthukile: Ihlanganisa ubuchwepheshe be-CHEF ne-PACE pulsed-field ukuze kuzuzwe imiphumela emihle ngemizila eqondile, engagobi.
• Ukulawula Okuzimele: Ifaka ama-electrode eplatinamu alawulwa ngokuzimela angu-24 (ububanzi obungu-0.5mm), futhi i-electrode ngayinye ishintshwa ngayodwana.
• Umsebenzi Wokubala Okuzenzakalelayo: Ihlanganisa okuguquguqukayo kokhiye abaningi njenge-voltage gradient, izinga lokushisa, i-engeli yokushintsha, isikhathi sokuqala, isikhathi sokuphela, isikhathi sokushintsha samanje, isikhathi sokusebenza esiphelele, i-voltage, kanye nokwamanje kwezibalo ezizenzakalelayo, ukusiza abasebenzisi ukufinyelela izimo zokuhlola ezilungile.
• I-Algorithm Eyingqayizivele: Isebenzisa i-algorithm eyingqayizivele yokulawula ukushaya kwenhliziyo ukuze kube nemiphumela engcono yokuhlukanisa, ihlukanise kalula phakathi kwe-DNA yomugqa neyindilinga, ngokuhlukaniswa okuthuthukisiwe kwe-DNA enkulu eyindilinga.
• Ukuzenzakalela: Irekhoda ngokuzenzakalelayo futhi iqale kabusha i-electrophoresis uma isistimu iphazamisekile ngenxa yokwehluleka kwamandla.
• Okulungisekayo Umsebenzisi: Ivumela abasebenzisi ukuthi bazibekele ezabo izimo.
• Ukuvumelana nezimo: Uhlelo lungakhetha ama-voltage gradients athile kanye nezikhathi zokushintsha kububanzi obuthile bosayizi we-DNA.
• Isikrini Esikhulu: Sifakwe isikrini se-LCD esingu-7-intshi ukuze sisebenze kalula, esifaka isilawuli sesofthiwe esiyingqayizivele sokusetshenziswa okulula nokulula.
• Ukutholwa kwezinga lokushisa: Ama-probe okushisa akabili athola ngokuqondile izinga lokushisa lebhafa enomkhawulo wephutha ongaphansi kuka-±0.5℃.
• Isistimu Yokujikeleza: Iza nesistimu yomjikelezo webhafa elawula ngokunembile futhi eqaphe izinga lokushisa lesixazululo se-buffer, iqinisekisa izinga lokushisa elingaguquki kanye nebhalansi ye-ionic phakathi ne-electrophoresis.
• Ukuphepha Okuphezulu: Kuhlanganisa nekhava yezokuphepha ye-acrylic ebonisa ngale enqamula amandla ngokuzenzakalelayo lapho iphakanyiswa, kanye nemisebenzi yokuvikela ukugcwala nokungalayishi.
• Izinga Elilungisekayo: Ithangi le-electrophoresis kanye ne-gel caster ifaka izinyawo ezilungisekayo ukuze zileveli.
• I-Mold Design: I-tank ye-electrophoresis yenziwe ngesakhiwo sesikhunta esihlanganisiwe ngaphandle kokubopha; i-electrode rack ifakwe ama-electrode eplatinamu angu-0.5mm, eqinisekisa ukuqina nemiphumela yokuhlola ezinzile.
FAQ
Q: Iyini iPulsed Field Gel Electrophoresis?
A: I-Pulsed Field Gel Electrophoresis iyindlela esetshenziselwa ukuhlukanisa ama-molecule amakhulu e-DNA ngokusekelwe kusayizi wawo. Kuhilela ukushintsha indlela yenkambu kagesi ku-matrix yejeli ukuze unike amandla ukuhlukaniswa kwezingcezu ze-DNA ezinkulu kakhulu ukuthi zingaxazululwa nge-electrophoresis yejeli ye-agarose.
Q: Yiziphi izicelo zePulsed Field Gel Electrophoresis?
A: I-Pulsed Field Gel Electrophoresis isetshenziswa kakhulu kubhayoloji yamangqamuzana nezakhi zofuzo:
Imephu yama-molecule amakhulu e-DNA, njengama-chromosome nama-plasmids.
• Ukunquma osayizi bofuzo.
• Ukutadisha ukuhlukahluka kofuzo kanye nobudlelwane bokuziphendukela kwemvelo.
• I-molecular epidemiology, ikakhulukazi ukulandelela ukuqubuka kwezifo ezithathelwanayo.
• Ukuhlaziywa kokulimala nokulungiswa kwe-DNA.
• Ukunquma ukuba khona kwezakhi zofuzo ezithile noma ukulandelana kwe-DNA.
Q: Isebenza kanjani iPulsed Field Gel Electrophoresis?
A: I-Pulsed Field Gel Electrophoresis isebenza ngokubeka ama-molecule e-DNA endaweni kagesi eshaywayo eshintshana lapho eya khona. Lokhu kuvumela ama-molecule amakhulu e-DNA ukuthi azihlele kabusha phakathi kwama-pulses, okwenza ukunyakaza kwawo ku-matrix yejeli. Ama-molecule e-DNA amancane ahamba ngokushesha ejeli, kuyilapho amakhulu ehamba kancane, okuvumela ukuhlukana kwawo ngokusekelwe kusayizi.
Q: Uyini umgomo ngemuva kwePulsed Field Gel Electrophoresis?
A: I-Pulsed Field Gel Electrophoresis ihlukanisa ama-molecule e-DNA ngokusekelwe kusayizi wawo ngokulawula ubude nokuqondisa kwama-pulses field kagesi. Inkambu eshintshanayo idala ama-molecule amakhulu e-DNA ukuthi aqhubeke nokuzihlela kabusha, okuholela ekufudukeni kwawo nge-matrix yejeli nokuhlukana ngokosayizi.
Q: Yiziphi izinzuzo ze-Pulsed Field Gel Electrophoresis?
A: Ukulungiswa okuphezulu kokuhlukanisa ama-molecule e-DNA amakhulu afinyelela ezigidini ezimbalwa zamapheya esisekelo.Ikhono lokuxazulula kanye nokuhlukanisa izingcezu ze-DNA ezinobukhulu obufanayo.Ukuguquguquka kokusebenza, kusukela ekuthayipheni kwe-microbial kuya kufuzo lwamamolekyuli kanye ne-genomics.Indlela esunguliwe yezifundo ze-epidemiological kanye nemephu yofuzo.
Q: Yiziphi izinto ezidingekayo ukuze iPulsed Field Gel Electrophoresis?
A: I-Pulsed Field Gel Electrophoresis ngokuvamile idinga i-electrophoresis apparatus enama-electrode akhethekile ukuze ikhiqize izinkambu ezishayekile. I-matrix yejeli ye-agarose enokugxilisa ingqondo efanele kanye nesigcinalwazi. Ukunikezwa kwamandla okukwazi ukukhiqiza i-high-voltage pulses.Isistimu yokupholisa yokukhipha ukushisa okukhiqizwa ngesikhathi se-electrophoresis, kanye nephampu yokujikeleza.
