I-PCR Thermal Cycler WD-9402M

I-Polymerase Chain Reaction (PCR) iyindlela yebhayoloji yamangqamuzana esetshenziswa kabanzi ekusetshenzisweni okuhlukahlukene. Ezinye izinhlelo zokusebenza ezijwayelekile ze-PCR zifaka:

I-DNA Amplification: Injongo eyinhloko ye-PCR ukukhulisa ukulandelana kwe-DNA ethile. Lokhu kubalulekile ekutholeni amanani anele e-DNA ukuze kuqhutshekwe nokuhlaziya noma ukuhlola.

Ukuhlolwa Kwezakhi Zofuzo: I-PCR isetshenziswa kakhulu ekuhlolweni kofuzo ukuhlonza izimpawu ezithile zofuzo noma izinguquko ezihlobene nezifo. Ibalulekile ngezinjongo zokuxilonga kanye nokutadisha izakhi zofuzo.

I-DNA Cloning: I-PCR isetshenziselwa ukukhiqiza amanani amakhulu ocezu oluthile lwe-DNA, olungase luhlanganiswe lube ivekhtha ukuze kuqhutshekwe nokusetshenziswa noma ukuhlaziya.

Ukuhlaziywa kwe-DNA ye-Forensic: I-PCR ibalulekile kwisayensi yezobunhloli ukuze kukhuliswe amasampula e-DNA atholakala ezigcawini zobugebengu. Kuyasiza ekuboneni abantu ngabanye nasekusunguleni ubudlelwano bofuzo.

Ukutholwa Kwemicrobial: I-PCR isetshenziselwa ukutholwa kwamagciwane amancane kumasampula omtholampilo noma amasampula emvelo. Ivumela ukuhlonza ngokushesha ama-ejenti athathelwanayo.

I-Quantitative PCR (qPCR noma i-PCR Yesikhathi Sangempela): I-qPCR inika amandla ukulinganisa kwe-DNA phakathi nenqubo yokukhulisa. Isetshenziselwa ukulinganisa amazinga wokuvezwa kofuzo, ukuthola inani legciwane, kanye nokulinganisa inani lokulandelana kwe-DNA ethile.

Izifundo Zokuziphendukela Kwemvelo: I-PCR isetshenziswa ezifundweni ezihlola ukuhlukahluka kofuzo phakathi kwabantu, ubudlelwano bokuvela kwemvelo, kanye nokuhlaziywa kwe-phylogenetic.

I-Environmental DNA (eDNA) Ukuhlaziywa: I-PCR iqashwe ukuze kutholwe ubukhona bezinto ezithile eziphilayo kumasampula emvelo, okunikela ezinhlobonhlobo zezinto eziphilayo kanye nezifundo zemvelo.

Ubunjiniyela Bofuzo: I-PCR iyithuluzi elibalulekile kubunjiniyela bezakhi zofuzo ukwethula ukulandelana kwe-DNA okuthile ezintweni eziphilayo. Isetshenziswa ekudaleni i-genetically modified organisms (GMOs).

Ukulandelanisa Ukulungiselelwa Kwelabhulali: I-PCR ibandakanyeka ekulungiseni imitapo yolwazi ye-DNA yobuchwepheshe bokulandelana kwesizukulwane esilandelayo. Isiza ukukhulisa izingcezu ze-DNA zezinhlelo zokusebenza zokulandelana komfula.

I-Mutagenesis Eqondiswe Kusayithi: I-PCR isetshenziselwa ukwethula izinguquko ezithile ekulandeleni kwe-DNA, okuvumela abacwaningi ukuthi bafunde imiphumela yezinguquko ezithile zofuzo.

I-DNA Fingerprinting: I-PCR isetshenziswa kumasu okunyathelisa iminwe e-DNA ukuze kuhlonzwe umuntu ngamunye, ukuhlolwa kobaba, kanye nokusungula ubudlelwano bebhayoloji.