I-PCR Thermal Cycler WD-9402D
Imininingwane
| Imodeli | I-WD-9402D |
| Amandla | 96×0.2ml |
| Ithubhu | Ishubhu engu-0.2ml, imichilo eyi-8, ipuleti lemithombo yesigamu sesiketi, Alikho ipuleti lemithombo ye-skirt engu-96 |
| Ivolumu yokusabela | 5-100ul |
| Izinga Lokushisa | 0-105 ℃ |
| MAX. I-Ramp Rate | 5℃/s |
| Ukufana | ≤±0.2℃ |
| Ukunemba | ≤±0.1℃ |
| Bonisa Ukulungiswa | 0.1℃ |
| Ukulawula izinga lokushisa | Block/Tube |
| I-Ramping Rate Iyalungiseka | 0.01-5℃ |
| I-Gradient Temp. Ibanga | 30-105 ℃ |
| Uhlobo Lwegradient | I-Gradient Evamile |
| Ukusabalala kweGradient | 1-42℃ |
| I-Hot Lid Temperature | 30-115 ℃ |
| Inombolo Yezinhlelo | 20000 +(USB FLASH) |
| Ubukhulu. Inombolo Yesinyathelo | 40 |
| Ubukhulu. Inombolo Yomjikelezo | 200 |
| Ukwenyuka/Ukunciphisa Isikhathi | 1 Isekhondi - 600 Isekhondi |
| Ukwenyuka Kwezinga Lokushisa/Ukuncipha | 0.1-10.0℃ |
| Misa Isikhashana Umsebenzi | Yebo |
| Ukuvikelwa Kwedatha Okuzenzakalelayo | Yebo |
| Bamba ku-4℃ | Phakade |
| Umsebenzi Wokuthinta | Yebo |
| Umsebenzi omude we-PCR | Yebo |
| Ulimi | IsiNgisi |
| Isoftware Yekhompyutha | Yebo |
| I-APP yeselula | Yebo |
| I-LCD | 10.1 intshi, 1280×800 amapels |
| Ukuxhumana | I-USB2.0 , i-WIFI |
| Ubukhulu | 385mm×270mm×255mm (L×W×H) |
| Isisindo | 10kg |
| Ukunikezwa kwamandla kagesi | 100-240VAC , 50/60Hz , 600 W |
Incazelo
I-thermal cycler isebenza ngokushisisa nokupholisa ngokuphindaphindiwe ingxube yokusabela equkethe isifanekiso se-DNA noma i-RNA, ama-primer, nama-nucleotide. Ukuhamba ngebhayisikili kwezinga lokushisa kulawulwa ngokunembile ukuze kuzuzwe izinyathelo ezidingekayo ze-denaturation, annealing, nezandiso zenqubo ye-PCR.
Ngokuvamile, umjikelezo oshisayo unebhulokhi equkethe imithombo eminingi noma amashubhu lapho ingxube yokusabela ibekwe khona, futhi izinga lokushisa emthonjeni ngamunye lilawulwa ngokuzimela. Ibhulokhi iyashisisa futhi ipholiswe kusetshenziswa i-Peltier element noma enye isistimu yokushisisa nokupholisa.
Amabhayisikili amaningi ashisayo anokusebenzelana okusebenziseka kalula okuvumela umsebenzisi ukuthi ahlele futhi alungise amapharamitha wokuhamba ngebhayisikili, njengezinga lokushisa le-anneal, isikhathi sokunwetshwa, kanye nenani lemijikelezo. Angase futhi abe nesibonisi sokuqapha ukuqhubeka kokusabela, futhi amanye amamodeli angase anikeze izici ezithuthukile ezifana nokulawula izinga lokushisa le-gradient, ukulungiselelwa kwamabhulokhi amaningi, nokuqapha nokulawula okukude.
Isicelo
I-Genome cloning; Ukulungiswa kwe-PCR ye-asymmetric ye-DNA enomucu owodwa wokulandelana kwe-DNA; I-PCR ephambene ukuze kunqunywe izifunda ze-DNA ezingaziwa; ukubhalwa kabusha kwe-PCR (RT-PCR). Ukuze kutholwe izinga lokuvezwa kofuzo kumaseli, kanye nenani legciwane le-RNA kanye nokuhlanganisa okuqondile kwe-cDNA ngezakhi zofuzo ezithile; ukukhulisa ngokushesha iziphetho ze-cDNA; ukutholakala kwezakhi zofuzo; ingasetshenziswa ekutholeni izifo ezibangelwa amagciwane kanye namagciwane; ukuxilongwa kwezifo zofuzo; ukuxilongwa kwezimila; ucwaningo lwezokwelapha olufana nobufakazi obubonakalayo be-forensic, abukwazi ukusetshenziswa ocwaningweni lwezokwelapha lwezokwelapha.
Okufakiwe
• Izinga lokushisa eliphezulu nokupholisa, ubuningi. Izinga lokugijima 8 ℃/s;
• Qala kabusha ngokuzenzakalelayo ngemva kokuhluleka kwamandla. Uma amandla ebuyisiwe ingaqhubeka nokusebenzisa uhlelo olungaqediwe;
• Umsebenzi wokufukamela osheshayo ngokuchofoza okukodwa ungahlangabezana nezidingo zokuhlola ezifana ne-denaturation, i-enzyme cutting/enzyme-link kanye ne-ELISA;
• Izinga lokushisa lesivalo esishisayo kanye nemodi yokusebenza yesivalo esishisayo ingasethwa ukuze ihlangabezane nezidingo zokuhlolwa okuhlukile;
• Isebenzisa amamojula e-Peltier empilo ende aqondene nokuhamba ngebhayisikili ;
• Imojula ye-aluminium anodized enokuqinisa ubunjiniyela, egcina ukusebenza kokushisa okusheshayo futhi enokumelana nokugqwala okwanele;
• Amazinga okushisa asheshayo erempu, anesilinganiso serempu esiphezulu esingu-5°C/s, okonga isikhathi sokuhlola esibalulekile;
• Ikhava eshisayo yesitayela se-Adaptive pressure bar, engavalwa ngokuqinile ngesinyathelo esisodwa futhi engakwazi ukuzivumelanisa nobude beshubhu obuhlukene;
• Idizayini yokugeleza komoya ngaphambili ukuya emuva, evumela imishini ukuthi ibekwe eceleni;
• Isebenzisa isistimu yokusebenza ye-Android, ehambisana nesikrini sokuthinta esingu-10.1-inch capacitive, esinesithombe esibonakalayo sokuzulazula sesitayela semenyu, okwenza umsebenzi ube lula kakhulu;
• Izifanekiso zefayela zohlelo ezakhelwe ngaphakathi eziyi-11, ezingahlela ngokushesha amafayela adingekayo;
• Ukuboniswa kwesikhathi sangempela kwenqubekelaphambili yohlelo nesikhathi esisele, ukusekela ukuhlelwa kwamaphakathi kwethuluzi le-PCR;
• Umsebenzi wokufukamela osheshayo onenkinobho eyodwa, uhlangabezana nezidingo zokuhlolwa okufana ne-denaturation, ukugaya/ukuxhunywa kwe-enzyme, kanye ne-ELISA;
• Izinga lokushisa elishisayo lekhava kanye nemodi yokusebenza yekhava eshisayo ingasethwa ukuze ihlangabezane nezidingo zokuhlola ezihlukene;
• Ukuvikelwa kokucisha amandla okuzenzakalelayo, ukwenza ngokuzenzakalelayo imijikelezo engaqediwe ngemva kokubuyiselwa kwamandla, okuqinisekisa ukusebenza okuphephile kuyo yonke inqubo yokukhulisa;
• Isixhumi esibonakalayo se-USB sisekela isitoreji/ukubuyisa idatha ye-PCR kusetshenziswa idrayivu ye-USB futhi singasebenzisa igundane le-USB ukulawula ithuluzi le-PCR;
• Isekela izibuyekezo zesofthiwe nge-USB ne-LAN;
• Imojula ye-WIFI eyakhelwe ngaphakathi, evumela ikhompuyutha noma umakhalekhukhwini ukuthi ulawule ngesikhathi esisodwa amathuluzi amaningi e-PCR ngoxhumano lwenethiwekhi;
• Isekela isaziso se-imeyili uma uhlelo lokuhlola luqedile.
FAQ
Umbuzo: Iyini ibhayisikili elishisayo?
A: I-thermal cycler idivayisi yaselabhorethri esetshenziselwa ukukhulisa ukulandelana kwe-DNA noma i-RNA ngokusebenzisa i-polymerase chain reaction (PCR). Isebenza ngokuhamba ngebhayisikili ngochungechunge lwezinguquko zokushisa, ivumela ukulandelana kwe-DNA okuqondile ukuthi kukhuliswe.
Q: Yiziphi izingxenye eziyinhloko zebhayisikili elishisayo?
A: Izingxenye eziyinhloko ze-thermal cycler zihlanganisa i-heater block, i-thermoelectric cooler, izinzwa zokushisa, i-microprocessor, kanye nephaneli yokulawula.
Umbuzo: Isebenza kanjani ibhayisikili elishisayo?
A: Ibhayisikili elishisayo lisebenza ngokushisisa nokupholisa amasampula e-DNA ochungechungeni lwemijikelezo yezinga lokushisa. Inqubo yokugibela ibhayisikili ibandakanya izigaba zokushintshashintsha kwe-denaturation, annealing, nezandiso, ngasinye sinezinga lokushisa elithile kanye nobude besikhathi. Le mijikelezo ivumela ukulandelana kwe-DNA ethile ukuthi kukhuliswe nge-polymerase chain reaction (PCR).
Q: Yiziphi izici ezibalulekile okufanele uzicabangele lapho ukhetha umjikelezo oshisayo? IMP: Ezinye izici ezibalulekile okufanele uzicabangele lapho ukhetha ibhayisikili elishisayo zihlanganisa inombolo yemithombo noma amashubhu okusabela, izinga lokushisa nesivinini serempu, ukunemba nokufana kokulawula izinga lokushisa, kanye nesixhumi esibonakalayo somsebenzisi namandla esofthiwe.
Q: Uyigcina kanjani ibhayisikili elishisayo?
IMP: Ukuze ulondoloze umjikelezo oshisayo, kubalulekile ukuhlanza njalo ibhulokhi yokushisisa namashubhu asabelayo, uhlole ukuguga nokukhala kwezingxenye, futhi ulinganise izinzwa zokushisa ukuze uqinisekise ukulawula izinga lokushisa okunembile nokungaguquguquki. Kubalulekile futhi ukulandela imiyalelo yomkhiqizi yokugcinwa nokulungiswa okujwayelekile.
Q: Yiziphi izinyathelo ezijwayelekile zokuxazulula inkinga zomshini oshisayo?
A: Ezinye izinyathelo ezijwayelekile zokuxazulula inkinga zomshini oshisayo zihlanganisa ukuhlola izingxenye ezixegayo noma ezilimele, ukuqinisekisa izinga lokushisa elifanele nezilungiselelo zesikhathi, nokuhlola amashubhu okusabela noma amapuleti ukuze angcole noma alimale. Kubalulekile futhi ukubhekisela emiyalweni yomkhiqizi ngezinyathelo ezithile zokuxazulula inkinga nezisombululo.
