Ithini inqubo yebhayisikili eshisayo ku-PCR?

I-Polymerase Chain Reaction (PCR) iyindlela yebhayoloji yamangqamuzana esetshenziselwa ukukhulisa izingcezu ezithile ze-DNA. Kungabhekwa njengendlela ekhethekile yokuphindaphinda kwe-DNA ngaphandle kwento ephilayo. Isici esiyinhloko se-PCR amandla ayo okwandisa kakhulu amanani omkhondo we-DNA.

Uhlolojikelele lwe-Polymerase Chain Reaction Cycle

Isithombe esivela ku-Bioninja

Ithini inqubo yebhayisikili eshisayo ku-PCR?

I-PCR inezinyathelo ezintathu eziyisisekelo:

1. Ukuhlukaniswa Kwe-DNA Yesifanekiso: I-DNA yesifanekiso ishisiswe ifike ku-93°C isikhathi esithile, okubangela ukuthi i-DNA enemicu ekabili ihlukane ibe imicu eyodwa, isilungiselela ukubopha i-primer emjikelezweni wokusabela olandelayo.

2. Ukufakwa Kwe-DNA Yesifanekiso Neziqalo: Ngemva kokukhishwa kwe-denaturation, izinga lokushisa liyehliselwa cishe ku-55°C, okuvumela ama-primers ukuthi abophe ukulandelana okuhambisanayo kusifanekiso esinomucu owodwa we-DNA.

3. Ukunwetshwa kwama-Primers: Ku-72°C, i-DNA template-primer complex inwetshwa ngosizo lwe-DNA polymerase (njenge-Taq DNA polymerase), kusetshenziswa ama-dNTP njengama-substrates ukuze kuhlanganiswe umucu omusha ohambisanayo ngokusekelwe kusifanekiso se-DNA.

Beijing LIUYI PCR Machine

Ngokuphinda izinqubo ze-denaturation, annealing, nezandiso, kukhiqizwa “amacu aphindaphindayo angawongi kangako”, angasebenza njengezifanekiso zemijikelezo elandelayo. Umjikelezo ngamunye uthatha cishe amaminithi angu-2 kuya kwangu-4, okuvumela isakhi sofuzo esiqondiwe ukuthi sithuthukiswe izikhathi eziyizigidi phakathi kwamahora angu-2 kuya kwangu-3.

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekile ekukhiqizeni amathuluzi e-electrophoresis iminyaka engaphezu kwengu-50 neqembu lethu lezobuchwepheshe eliqeqeshiwe kanye nesikhungo se-R&D. Sinomugqa wokukhiqiza onokwethenjelwa nophelele kusukela ekuklanyweni kuya ekuhlolweni, kanye nendawo yokugcina impahla, kanye nokwesekwa kokumaketha. Imikhiqizo yethu eyinhloko i-Electrophoresis Cell (ithangi/igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njll. Siphinde sinikeze amathuluzi aselabhu njenge-PCR instrument, i-vortex mixer kanye ne-centrifuge yaselabhorethri.

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