Izinyathelo Zokusebenza Ze-Semi-dry Trans Blot Apparatus DYCP-40C

I-DYCP-40C semi-dry blotting system isetshenziswa kanye namandla e-electrophoresis ukudlulisa amaprotheni kumajeli e-polyacrylamide kulwelwesi olufana nolwelwesi lwe-nitrocellulose, ulwelwesi lwenayiloni kanye nolwelwesi lwe-PVDF. Ukusula kancane kancane kwenziwa ngama-electrode e-graphite plate ngendlela evundlile, kuhlanganiswe ijeli nolwelwesi phakathi kwamashidi ephepha lesihlungi elifakwe isiphazamiso elisebenza njengechibi le-ion. Ngesikhathi sokudluliswa kwe-electrophoretic, ama-molecule anecala elibi aphuma kujeli futhi adlulele ku-electrode ephozithivu, lapho efakwa khona kulwelwesi. Ama-electrode amapuleti, ahlukaniswe kuphela ijeli nesitaki sephepha lesihlungi, anikeza amandla ensimu aphezulu (V/cm) kuyo yonke ijeli, enza ukudluliselwa okuphumelelayo kakhulu, okusheshayo. Indawo yokudlulisa yeseli encane ye-DYCP - 40C electrophoresis ingu-150 × 150 (mm), ifanele ukudlulisa ama-gel ajwayelekile, kuhlanganise nalawo asuka ku-DYCZ-24DN kanye ne-DYCZ-24EN electrophoresis cell.

Ake sifunde kabanzi mayelana nokusebenza kwalesi sisetshenziswa se-semi-dry trans blot.

Izinto, amathuluzi okusebenza i-DYCP-40C
Ukunikezwa kwamandla kwe-Electrophoresis i-DYY-6C, i-semi-dry trans blot apparatus i-DYCP-40C, isisombululo se-buffer, neziqukathi zesixazululo se-buffer. njll.

Izinyathelo Zokusebenza
1. Faka ijeli namapuleti engilazi ekhambi lokudlulisa

2. Kala usayizi wejeli

3.Lungiselela izingcezu ezi-3 zephepha lokuhlunga ngokosayizi wejeli, futhi usayizi wephepha lokuhlunga kufanele ube mkhulu kunosayizi wejeli; Lapha sisebenzisa iphepha lokuhlunga le-Whatman;

4.Faka izingcezu ezi-3 zephepha lokuhlunga kwikhambi le-buffer kancane, bese uvumela iphepha lokuhlunga licwilise ku-buffer ngokuphelele, futhi ugweme ukuba namabhamuza omoya;

5.Lungiselela futhi usike ulwelwesi lwe-nitrocellulose ngokuya ngosayizi wejeli kanye nephepha lokuhlunga; ubukhulu be-nitrocellulose membrane kufanele bube bukhulu kunobukhulu bejeli nephepha lokuhlunga;

6.Faka ulwelwesi lwe-nitrocellulose kusixazululo se-buffer;

7.Khipha izingcezu ezi-3 zephepha lokuhlunga bese ulahla isisombululo se-buffer eyengeziwe kuze kube yilapho isisombululo se-buffer siphuma kulwelwesi; bese ubeka iphepha lokuhlunga ngaphansi kwe-DYCP-40C;

8.Thatha ijeli emapuletini engilazi, hlanza ngobumnene ijeli yokupakisha, bese ufaka ijeli kusixazululo se-buffer;

9.Beka ijeli ephepheni lokuhlunga, qala kusukela ekupheleni kwejeli ukuze ugweme ama-bubbles emoyeni;

10.Sebenzisa ithuluzi elifanele ukususa amabhamuza omoya phakathi kwejeli nephepha lokuhlunga.

11.Vala ulwelwesi lwe-nitrocellulose kujeli, uhlangothi olumaholoholo olubheke kujeli. Bese usebenzisa ithuluzi elifanele ukususa amabhamuza omoya phakathi kwe-membrane nejeli. Faka izingcezu ezi-3 zephepha lokuhlunga kulwelwesi. Kusadingeka usebenzise ithuluzi elifanele ukususa amabhamuza omoya phakathi kwephepha lokuhlunga kanye nolwelwesi.

12.Vala isembozo, bese usetha imingcele esebenzayo ye-electrophoresis, i-80mA yamanje engashintshi;

13.I-electrophoresis yenziwa. Sithola umphumela njengokulandelayo;

I-Beijing Liuyi Biotechnology Co., Ltd ibikhethekile ekukhiqizeni imikhiqizo ye-electrophoresis iminyaka engaphezu kwengu-50. Siyinkampani eqinisekisiwe ye-ISO9001 ne-ISO13485 futhi sisebenza ngokukhethekile ekukhiqizeni amathangi e-electrophoresis, izinsiza zikagesi, i-UV transilluminator, kanye nejeli yemibhalo kanye nohlelo lokuhlaziya, okwamanje, sihlinzeka ngesevisi ye-OEM kumakhasimende ethu, kanye nesevisi ye-ODM.

Manje sifuna ozakwethu, kokubili i-OEM nabasabalalisi bamukelekile.

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