Ingabe unabo ubunzima ekulungiseni ijeli ye-agarose?Ake silandeleuchwepheshe wethu welebhu ekulungiseni ijeli.
Inqubo yokulungiselela ijeli ye-agarose ihlanganisa lezi zinyathelo ezilandelayo:
Isisindo se-Agarose Powder
Kala inani elidingekayo lempushana ye-agarose ngokuya ngokugxila okufunayo kokuhlolwa kwakho. Ukugxila kwe-agarose okuvamile kusuka ku-0.5% kuya ku-3%. Ukugxila okuphezulu kusetshenziselwa ukuhlukanisa ama-molecule amancane e-DNA, kuyilapho ukugxila okuphansi kungama-molecule amakhulu.
Ilungiselela Isixazululo Sebhafa
Engeza impushana ye-agarose kusigcinalwazi esifanelekile se-electrophoresis, njenge-1× TAE noma 1× TBE. Ivolumu yebhafa kufanele ifane nevolumu yejeli edingekayo ekuhloleni kwakho.
Ukuqothula i-Agarose
Shisisa ingxube ye-agarose ne-buffer kuze kube yilapho i-agarose ichitheka ngokuphelele. Lokhu kungenziwa ngokusebenzisa i-microwave noma ipuleti elishisayo. Govuza isixazululo ngezikhathi ezithile ukuze ungabilisi. Isixazululo se-agarose kufanele sicace ngaphandle kwezinhlayiya ezibonakalayo.
Ukupholisa Isixazululo se-Agarose
Vumela isisombululo se-agarose esishisayo siphole sibe ngu-50-60 ° C. Gcoba ikhambi phakathi nenqubo yokupholisa ukuze uvimbele ukuqina ngaphambi kwesikhathi.
Ukungeza i-Nucleic Acid Stain (Ongakukhetha)
Uma ufuna ukubona ngeso lengqondo i-DNA noma i-RNA kujeli, ungakwazi ukwengeza ibala le-nucleic acid, njenge-GelRed noma i-ethidium bromide, kulesi sigaba. Lapho uphatha lawa mabala, gqoka amagilavu futhi usebenzise ukuqapha, njengoba angaba nobuthi.
Ukuphonsa iGel
Thela isisombululo se-agarose esipholile esikhunjini sejeli se-electrophoresis esilungisiwe. Faka ikamu ukuze udale imithombo yesampula, uqinisekise ukuthi ikamu livikelekile futhi isisombululo sisabalaliswa ngokulinganayo esibunjeni.
I-gel Solidification
Vumela ijeli ukuthi iqine ekamelweni lokushisa, okuvame ukuthatha imizuzu engu-20-30 kuye ngokugxila nokuqina kwejeli.
Rekhipha iComb
Uma ijeli seliqinile ngokugcwele, khipha ngokucophelela ikamu ukuze uveze imithombo yesampula. Beka ijeli kanye nesikhunta ekamelweni le-electrophoresis bese ulimboza ngenani elifanele le-electrophoresis buffer, uqinisekise ukuthi ijeli icwiliswe ngokuphelele.
Ukulungiselela i-Electrophoresis
Ngemuva kokuthi ijeli isilungile, layisha amasampula akho emithonjeni, futhi uqhubeke nokuhlolwa kwe-electrophoresis.
Uma unezinkinga ngokulungiswa kwejeli, zizwe ukhululekile ukuxhumana nathi. Sinochwepheshe belebhu abatholakalayo ukuze baxhumane nawe.
Sijabule ukwabelana ngezindaba ezinhle: ithangi lethu le-DYCP-31DN elivundlile le-electrophoresis liyanyuswa okwamanje., xhumana nathi ukuze uthole ulwazi olwengeziwe manje!
I-DYCP-31DN ithangi le-electrophoresis elivundlile
I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekile ekukhiqizeni amathuluzi e-electrophoresis iminyaka engaphezu kwengu-50 neqembu lethu lezobuchwepheshe eliqeqeshiwe kanye nesikhungo se-R&D. Sinomugqa wokukhiqiza onokwethenjelwa nophelele kusukela ekuklanyweni kuya ekuhlolweni, kanye nendawo yokugcina impahla, kanye nokwesekwa kokumaketha. Imikhiqizo yethu eyinhloko i-Electrophoresis Cell (ithangi/igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njll. Siphinde sinikeze amathuluzi aselabhu njenge-PCR instrument, i-vortex mixer kanye ne-centrifuge yaselabhorethri.
Uma unecebo lokuthenga lemikhiqizo yethu, sicela ungangabazi ukusithinta. Ungasithumela umlayezo nge-imeyili[i-imeyili ivikelwe]noma[i-imeyili ivikelwe], noma sicela usishayele ku- +86 15810650221 noma wengeze uWhatsapp +86 15810650221, noma Wechat: 15810650221.
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Isikhathi sokuthumela: Oct-15-2024