I-Protein Electrophoresis Common Issues (2)

Sabelane ngezinkinga ezivamile mayelana namabhendi e-electrophoresis ngaphambili, futhi nathi'd ngithanda ukwabelana ngezinye izinto ezingavamile ze-polyacrylamide gel electrophoresis ngakolunye uhlangothi. Thinafinyeza lezi zinkinga kumakhasimende ethu' ireferensi yokuthola izizathu kanye nokuthola imiphumela enembile futhi uthuthukise ikhwalithi yokuhlukaniswa kwamaprotheni.

Ijeli eyenziwe nge-polymerized Kungenzeka

Izizathu yilezi:

(a) Ukuhlanzeka kwe-monomer okunganele, okudinga ukwenziwa kabusha kwekristalu.

(b) I-oksijini encibilikisiwe kusixazululo sejeli kuvimbela ukupholisa kwejeli. Kufanele kusetshenziswe iphampu yokukhipha amanzi esebenzayo.

(c) I-Ammonium persulfate ayisebenzi noma ayanele ngobuningi. Lungisa kabusha noma usebenzise enye inqwaba ye-ammonium persulfate enokugxila okuphezulu.

 Tijeli isivele i-polymerized ngaphandle kokwengeza ungqimba lwamanzi.

Izizathu yilezi:

(a) Pholisa isixazululo sejeli ngaphambi kokwengeza i-catalyst ukuze ubambezele izinga le-polymerization.

(b) Yehlisa inani le-TEMED noma i-ammonium persulfate esetshenzisiwe.

(c) Sheshisa ukuhlinzwa.

Ijeli iyaslayida ngaphandle kwegumbi le-electrophoresis ngemva kokwenziwa kwe-polymerization

Lokhu kujwayele ukwenzeka kuma-gel ane-concentration ephansi. Isixazululo esisodwa ukusonga ulwelwesi lwe-dialysis ngaphansi kweshubhu noma usebenzise isisekelo se-porous polypropylene.

Isampula ayitholakali ngemuva kwe-electrophoresis

Izizathu yilezi:

(a) Inani lesampula elinganele. Nyusa inani lesampula.

(b) Izakhiwo zesixazululo sokugcoba noma ukugxilisa ingqondo noma isikhathi sokufaka amabala azanele. Faka esikhundleni isixazululo sokugcoba futhi ukwandise ukugxila kanye nesikhathi sokugcoba.

(c) Isampula lintante laphuma ngesikhathi lilayishwa. Khulisa ukuminyana kwesixazululo sesampula futhi ubambe ngokucophelela.

(d) Ukugxiliswa kwejeli yokuhlukanisa kuphezulu kakhulu, futhi isampula ayizange ingene kujeli. Lungisa ukuhlushwa kwejeli ngendlela efanele.

(e) Ukugxiliswa kwejeli yokuhlukanisa kuphansi kakhulu, futhi isampuli ikhishwe nge-electrophores ngaphandle kwejeli yokuhlukanisa. Lungisa ukuhlushwa kwejeli kanye nezimo ezifanele ze-electrophoresis.

(f) Uma isampula kuyi-RNA, ingase iqukathe amaprotheni akha inkimbinkimbi enkulu evimba izimbotshana zejeli. Susa ngokuphelele amaprotheni kusampula ye-RNA.

(g) Isampula iqukethe ama-enzyme enza isampula i-hydrolyze, futhi isampuli yonakalisa ngesikhathi se-electrophoresis. Hlanza ngokuphelele isampula.

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I-Beijing Liuyi Biotechnology Company Ltd yenza uchungechunge lwemikhiqizo ye-electrophoresis engasiza ekuxazululeni izinkinga okungenzeka sihlangane nazo ekuhloleni kwethu i-electrophoresis. Le nkampani yasungulwa ngo-1970. Yayingeyesizwe futhi yakhiqiza umshini wokushisela kagesi kanye nemitha yokugeleza kwezimboni ngaleso sikhathi. Kusukela ngo-1979, iBeijing Liuyi Biotechnology Company Ltd iqala ukukhiqiza imikhiqizo ye-electrophoresis. Manje inkampaniis omunye wabaphambiliumkhiqizi wemishini yaselabhorethri nezinsimbi zesayensi ezinze eBeijing, eChina. Yakho uphawu lokuhwebaLIUYI idumile eChina kule ndawo.

Imikhiqizo yenkampani ihlanganisa izinhlobonhlobo zezinsimbi zaselabhorethri, okuhlanganisa ithangi le-nucleic acid electrophoresis evundlile, ithangi/iyunithi yephrotheni eqondile, ibhokisi elimnyamauhlobo I-UV analyzer,I-Gel Document Tracking Imaging Analyzer, kanye nogesi we-electrophoresis. Le mikhiqizo isetshenziswa kakhulu ezikhungweni zocwaningo, emanyuvesi, nasezimbonini ezahlukahlukene, njengezokwelapha, i-biotechnology, nokuqapha indawo ezungezile.Le nkampani iyinkampani eqinisekisiwe ye-ISO9001 & ISO13485 futhi inezitifiketi ze-CE.

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Banguizinhlobo ezahlukenempoamathangi e-electrophoresisamaprotheni electrophoresisukuhlaziya kanye nokuhlonza amasampula amaprotheni nge-polyacrylamide gel electrophoresis,futhifuthi ukuze kukale isisindo samangqamuzana amasampula, ukuhlanza amasampula kanye nokulungiselela amasampula.Le mikhiqizo yamukelwa yonkeimakethe yasekhaya neyaphesheya.

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Ukunikezwa kwamandla kwe-electrophoresis kuyisici esibalulekileisistimu ye-electrophoresis, ehlinzeka ngomthombo ozinzile futhi onembile wamanje kagesi ukushayela inqubo yokuhlukanisa.Itngokuvamile iletha i-voltage engaguquki noma yamanje engaguquki ohlelweni lwe-electrophoresis, kuye ngephrothokholi yokuhlola ethile. Iphinde ivumele umsebenzisi ukuthi alungise i-voltage noma okukhiphayo kwamanje, kanye namanye amapharamitha afana nesikhathi nezinga lokushisa, ukuze alungiselele izimo zokuhlukanisa zokuhlola okuthile.

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Fnoma ngokubuka ijeli, ungakhetha uchungechunge lwe-UV Transilluminator WD-9403 olukhiqizwa yi-Beijing Liuyi Biotechnology.A I-UV transilluminator iyithuluzi laselabhorethri elisetshenziselwa ukubona ngeso lengqondo nokuhlaziya i-DNA, i-RNA, namasampuli amaprotheni. Isebenza ngokukhanyisa amasampuli ngesibani se-UV, okwenza amasampuli akhanye futhi abonakale. Kunamamodeli amaningana we-UV transilluminatorokunikezwa yithi. I-WD-9403A iqondene ngokukhethekile nokubheka i-protein electrophoresis, kanti i-WD-9403F isetshenziselwa ukubuka i-DNA kanye ne-protein electrophoresis.

Lolu chungechunge lwemikhiqizo lungasebenza kusukela ekubunjweni kwejeli kuye ekuhloleni ijeli ngokuya ngezidingo zakho zokuhlola.Sazise izidingo zakho, kokubili i-OEM, i-ODM nabasabalalisi bamukelekile.Wsizozama konke okusemandleni ethu ukukunikeza imikhiqizo namasevisi ethu.

Uma unecebo lokuthenga lemikhiqizo yethu, sicela ungangabazi ukusithinta. Ungasithumela umlayezo nge-imeyili[i-imeyili ivikelwe]noma[i-imeyili ivikelwe], noma sicela usishayele ku- +86 15810650221 noma wengeze uWhatsapp +86 15810650221, noma Wechat: 15810650221.


Isikhathi sokuthumela: May-23-2023