Isingeniso Somkhiqizo: I-Agarose Gel Electrophoresis Tank DYCP-31DN

Uhlolojikelele olufushanekweI-Agarose Gel Electrophoresis

I-Agarose gel electrophoresis iyindlela esetshenziswa kakhulu kubhayoloji yamangqamuzana ekuhlukaniseni ama-nucleic acid, njenge-DNA ne-RNA, ngokusekelwe kusayizi wawo. Le ndlela isebenzisa ijeli eyenziwe nge-agarose, i-polysaccharide yemvelo etholakala olwandle. Ijeli isebenza njengesisefo samangqamuzana, ivumela ama-molecule ukuba afuduke kuyo lapho kusetshenziswa umsinga kagesi. Le nqubo yokuhlukanisa isiza abacwaningi ukuthi bahlaziye futhi baveze amasampula e-nucleic acid ngokubheka amabhande ahlukene akhiwe ngokosayizi bawo. I-Agarose gel electrophoresis iyithuluzi eliyisisekelo lemisebenzi efana nokuhlaziywa kweziqephu ze-DNA, ukuhlola ubumsulwa, nocwaningo lwebhayoloji yamangqamuzana.

Imvamisa, ithangi le-agarose gel electrophoresis libizwa nge-horizontal electrophoresis tank futhi. Izicelo ezinkulu ze-agarose gel electrophoresis zihlanganisa:

Ukuhlaziywa Kwengxenye Ye-DNA:

Ukuhlukaniswa nokuhlaziywa kwezingcezu ze-DNA ngokusekelwe kusayizi, kubalulekile emisebenzini efana ne-genotyping kanye ne-DNA fingerprinting.

Ukuhlaziywa kwe-RNA:

Ukuxazululwa kwama-molecule e-RNA, kusiza ocwaningweni lwamaphethini wokuvezwa kofuzo nobuqotho be-RNA.

Ukuqinisekiswa Komkhiqizo we-PCR:

Ukuqinisekiswa kwemikhiqizo ye-polymerase chain reaction (PCR) ukuze kuqinisekiswe ukukhulisa ngempumelelo nokuthola osayizi beziqephu ezinembile.

Ukuhlaziywa kwe-Plasmid DNA:

Ukuhlolwa kwe-plasmid DNA yokulawulwa kwekhwalithi, ukuqinisekiswa kokuhlolwa kwe-cloning, kanye nokuhlonzwa kwe-DNA ehlangene.

Ukuhlola Ukuhlanzeka:

Ukunqunywa kokuhlanzeka kwamasampuli e-nucleic acid ngokubona ngeso lengqondo ukungcola okungaba khona noma ukungcola.

Ucwaningo lwebhayoloji yamangqamuzana:

Ithuluzi eliyisisekelo kumalabhorethri ebhayoloji yamangqamuzana okuhlola okuhlukahlukene, okuhlanganisa amalungiselelo okulandelana kwe-DNA nokugaya ama-enzyme okukhawulela.

I-Beijing Liuyi Biotechnology Co., Ltd. (eyayiyi-Beijing Liuyi Instrument Factory) yasungulwa ngo-1970. Kuyibhizinisi lobuchwepheshe elisebenza ngokukhethekile ekukhiqizeni amathuluzi okuhlaziya amalabhorethri ezinto eziphilayo namangqamuzana ebhayoloji. ULiuyi ugxile ekwenzeni izinsimbi ze-electrophoresis iminyaka engaphezu kwengu-50 , futhi inomugqa wokukhiqiza onokwethenjelwa nophelele kusukela ekuklanyweni kuya ekuhlolweni, kanye nendawo yokugcina impahla, kanye nokwesekwa kokumaketha. Imikhiqizo eyinhloko i-Electrophoresis Cell (ithangi/igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, iGel Image & Analysis System njll.

Sazise okwengeziwe ngemodeli yayo eyodwa ye-agarose gel electrophoresis-DYCP-31DN.

Isingeniso sika ai-garose gel electrophoresisithangi le-DYCP-31DN

I-DYCP-31DN ingeyethangi le-agarose gel electrophoresis lika-Liuyi, elinomshini wokulingisa otholakalayo wokuphonsa izingcezu zejeli eziyi-1-4. Ingenza amajeli amabili amancane njengosayizi 60x60mm kanye nejeli eyodwa ebanzi engu-120x60mm noma ijeli ende engu-60x120mm ngesikhathi esifanayo. Ijeli enkulu kunazo zonke engakwazi ukuyisakaza ubukhulu buka-120x120cm.

Iukujiya kwekamu okunikelwekukhona1.0mm, 1.5mm kanye 2.0mm. Ngomklamo we-electrode ekhishwayo, kulula kakhulu ukuhlanza nokugcina. "Umklamo webhuloho" okuhloswe ngawo ukulondoloza isisombululo se-buffer ngesikhathi senqubo ye-electrophoresis. Ukusetshenziswa kwebhafa okusebenzayo kubalulekile ekugcineni izinkundla zikagesi ezizinzile kanye nokuqinisekisa ukuhlukaniswa okunembile kwama-molecule.

Amanye amakusasa abalulekile:

1) Izivalo kanye nemizimba yamathangi amakhulu (amathange ayisivikelo) asobala, abunjiwe, abukeka kahle, aqinile, aqinile, awangcolisi amakhemikhali;ayikwazi ukumelana namakhemikhali, ayizwani nokucindezela.

2) Ama-Electrode enziwa ngeplatinamu emsulwa(i-quotient yobumsulwa bensimbi ehlonishwayo ≥99.95%) enezici zokumelana nokugqwala kwe-electroanalysis futhi imelana nokushisa okuphezulu, umsebenzi wokuqhuba ugesi muhle kakhulu.

3) Isampula imithombo ngokuvamile kunzima ukubona, ngakho-ke ibhande elimnyama kuthreyi yejeli likwenza kube lula ukubuka nokulayisha amasampula.

I-4) Amandla ayahluleka uma uvula isivalo ukuze uthole isisefo somsebenzisi.

Kunamamodeli amaningana okunikezwa kwamandla okunikelwe i-DYCP-31DN. I-electrophoresis eshisa kakhulu ukunikezwa kwamandla kagesi is I-DYY-6C. Siyakwamukela ukuvakashela iwebhusayithi yethu ukuze uthole amamodeli engeziwe wokuphakelwa kwamandla.

Ukuze ubone ngeso lengqondo tyenaijeli ye-agarose ngeI-DNA noma i-RNA, uLiuyi uhlinzeka ngama-transilluminator e-UV kumakhasimende.I-WD-9403C, I-WD-9403B futhiI-WD-9403X zonke ziyatholakala ukuze ikhasimende lizikhethe. 

I-Agarose gel electrophoresis iwusizo emisebenzini efana nokuhlola usayizi wezingcezu ze-DNA, ukuhlola ukuhlanzeka kwesampula ye-DNA, noma ukuhlukanisa izingcezu ze-DNA ezihlukene ukuze kuhlaziywe okwengeziwe.

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