Isimiso se-Electrophoresis kanye Nezicelo Zaso Kusayensi Yezinto Eziphilayo

I-Electrophoresis iyindlela esetshenziselwa ukuhlukanisa ama-biomolecules ngokusekelwe kusayizi nokushaja kwawo kusetshenziswa inkambu kagesi. Isetshenziswa kabanzi kusayensi yezinto eziphilayo ngezinjongo ezihlukahlukene, kusukela ekuhlaziyweni kwe-DNA kuya ekuhlanzeni amaprotheni. Lapha, sihlola isimiso se-electrophoresis kanye nezinhlelo zokusebenza ezihlukahlukene.

Isimiso se-Electrophoresis

I-Electrophoresis incike ekuhambeni kwezinhlayiya ezishajiwe endaweni kagesi. Ukusetha okuyisisekelo kuhilela ukubeka isampula (equkethe ama-biomolecules ashajiwe) kujeli noma esixazululweni, kanye nokusebenzisa amandla kagesi. Ama-biomolecules ahamba phakathi nendawo ngamanani ahlukene asekelwe eshadini kanye nosayizi, okuholela ekwehlukaneni.

Izinhlobo ze-Electrophoresis

1. Gel Electrophoresis

I-Agarose Gel Electrophoresis: Ihlukanisa izingcezu ze-DNA ne-RNA ngokusekelwe kusayizi.

I-Polyacrylamide Gel Electrophoresis (IKHASI): Ixazulula amaprotheni ngokusekelwe kusayizi nokushaja.

2. I-Capillary Electrophoresis

Isebenzisa ama-capillary amancane ukuze ahlukanise, okuvumela ukuhlaziya okusheshayo kwe-DNA, i-RNA, namaprotheni.

Izicelo ku-Biological Science

1. Ukuhlaziywa KweDNA

I-Genotyping: Ikhomba ukuhlukahluka kofuzo (isb, ama-SNP) ahlobene nezifo.

I-DNA Sequencing: Inquma ukuhleleka kwama-nucleotide ku-molecule ye-DNA.

Ukuhlaziywa Kwe-DNA Fragment: Osayizi bezingxenye ze-DNA zokusetshenziswa kubhayoloji yamangqamuzana.

2. Ukuhlaziywa kwe-RNA

I-RNA Electrophoresis: Ihlukanisa ama-molecule e-RNA ukuze kuhlaziywe isisho sofuzo nobuqotho be-RNA.

3. Ukuhlaziywa Kwamaprotheni

I-SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis): Ihlukanisa amaprotheni ngokusekelwe kusayizi.

I-2D Electrophoresis: Ihlanganisa ukugxila kwe-isoelectric kanye ne-SDS-PAGE ukuze ihlukanise amaprotheni ngokusekelwe endaweni kanye nosayizi we-isoelectric.

4. Ukuhlanzwa

I-Electrophoresis Elungiselelayo: Ihlanza ama-biomolecules (isb, amaprotheni) ngokusekelwe enkokhelweni kanye nosayizi.

5. Izicelo Zomtholampilo

I-Hemoglobin Electrophoresis: Ihlola i-hemoglobinopathies (isb, isifo se-sickle cell).

I-Serum Protein Electrophoresis: Ikhomba okungavamile kumaprotheni e-serum.

6. Izicelo Zomthetho

I-DNA Profileing: Ifanisa amasampula e-DNA ophenyo lwe-forensic.

Izinzuzo ze-Electrophoresis

Ukulungiswa Okuphezulu: Ihlukanisa ama-biomolecules ngokusekelwe kusayizi nokushaja ngokunemba okuphezulu.

I-Versatility: Isebenza ku-DNA, i-RNA, amaprotheni, namanye ama-biomolecules ashajiwe.

Ukuhlaziywa Kobuningi: Ikala amanani ama-biomolecules ngokusekelwe kubukhulu bebhendi.

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekile ekukhiqizeni amathuluzi e-electrophoresis iminyaka engaphezu kwengu-50 neqembu lethu lezobuchwepheshe eliqeqeshiwe kanye nesikhungo se-R&D. Sinomugqa wokukhiqiza onokwethenjelwa nophelele kusukela ekuklanyweni kuya ekuhlolweni, kanye nendawo yokugcina impahla, kanye nokwesekwa kokumaketha. Imikhiqizo yethu eyinhloko i-Electrophoresis Cell (ithangi/igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njll. Siphinde sinikeze amathuluzi aselabhu njenge-PCR instrument, i-vortex mixer kanye ne-centrifuge yaselabhorethri.

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