Ukulungiselela i-Agarose Gel Ye-Electrophoresis

Ukulungiswa kwe-Agarose Gel ye-Electrophoresis

Qaphela: Gqoka njalo amagilavu ​​alahlwayo!

Isinyathelo Ngesinyathelo Imiyalelo

Isisindo se-Agarose Powder:usephepha lokukala nebhalansi ye-elekthronikhi ukukala u-0.3g wempushana ye-agarose (ngokusekelwe ohlelweni lwe-30ml).

Ilungiselela TBST Buffer:plungisa u-30ml we-1x TBST buffer ku-100ml Erlenmeyer flask.

Ukuncibilikisa i-Agarose Powder:pyethu impushana ye-agarose ku-buffer ye-TBST futhi unyakazise kahle.Bafakekuhhavini ye-microwave nokushisa (imvamisa imizuzwana engu-50, embozwe nge-aluminium foil) kuze kuqedwe ngokuphelele.

Ukupholisa kanye Nokwengeza Nuclease:use amagilavu ​​ukuze ukhiphe ingxube kuhhavini ye-microwave bese uyiyeka iphole kancane emanzini abandayo ize ifudumale (cishe ama-60°C). Engeza u-2µl we-nuclease (i-Eb esikhundleni) ngenkathi unyakazisa ukuze uhlanganise kahle.

Ukulungiselela i-Gel Mold:

• Cncika futhi omise iithreyi yejeli kanye nedivayisi yokuphonsa ijeliethangini le-electrophoresis.
•  Pi-lace ijeliugqokoethangini elingaphakathi bese ufaka ikama endaweni engashintshi.
• Hlanganisa isisombululo sejeli ye-agarose, epholile cishe ku-65 ° C, bese uyithela ngokucophelelaithreyi yejelikweidivayisi yokukhipha i-gel, isakaze kancane kancane ize yakha ungqimba lwejeli elilinganayo epuleti lengilazi.
• Yivumele ukuthi ihlale ekamelweni lokushisa kuze kube yilapho ijeli iqina ngokuphelele.
• Khipha ngobumnene ikama mpo bese ukhipha itheyiphu.
• Beka ijeliugqokoethangini le-electrophoresis.

Okubalulekile: Qinisekisa ukuthi awekho amabhamuza omoya endaweni yamazinyo ekama. Ubuso be-liquid kufanele busheleleke ngaphandle kwama-ripples.

Ukugijima iGel

Ilayisha iGel

Ngemuva kokuthi ijeli liqina, libeke ethangini le-electrophoresis bese uthela isigcinalwazi se-electrophoresis kuze kube yilapho ijeli icwiliswa emanzini.

Ukulungiselela Amasampula

• Thatha umaka futhi ulayishe isigcinalwazi esiqandisini.
• Engeza u-6µl webhafa yokulayisha kumasampuli bese uhlanganise kahle.
• Usebenzisa i-micropipette, layisha kancane amasampula emithonjeni emikhulu yejeli (qaphela ukuthi ungabhobozi ijeli futhi ungawukhiphi wonke umthamo ukuze ugweme amabhamuza omoya).
• Layisha umaka kunoma yimiphi imithombo emincane (khumbula indawo yayo).

Ukuqala kwe-Electrophoresis

• Vala ithangi le-electrophoresis bese uqala i-electrophoresis ngokushesha ngemva kokulayisha ijeli.
• Setha i-voltage ku-60-100V. Amasampuli azosuka ku-electrode engeyinhle (emnyama) aye ku-electrode ephozithivu (ebomvu).
• Amandla kagesi aphezulu afinyeza uhla lokuhlukanisa olusebenzayo lwejeli ye-agarose.
• Misa i-electrophoresis lapho idayi eluhlaza ye-bromophenol ifinyelela cishe ku-1cm ukusuka emaphethelweni aphansi epuleti lejeli.

Ukubuka Imiphumela

Ngemuva kokuhlukanisa ama-band, yeka i-electrophoresis, khipha i-gel, futhi uyibone ngokuqondile futhi uyithwebule.

Sebenzisa isistimu yokuthwebula ijeli ukuze uthwebule futhi ubheke amabhande (bheka ukuthi akhona yini amabhande omaka noma amasampula).

Ngemva kokuthola imephu yebhendi yejeli yakho, thola umaka. Ngokusekelwe kumaka, unganquma amabhendi okuqondiwe!

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) yenziwe imikhiqizo ye-electrophoresis iminyaka engaphezu kwengu-50. Sethula izinhlelo zethu ze-electrophoresis ezivundlile lapha ukuzeijeli ye-agarosei-electrophoresis.Khethaamasistimu we-electrophoresis ovundlilekusuka e-Beijing liuyi ngokuhlolwa kwakho kwe-gel electrophoresis ukusiza ucwaningo lwakho embonini ye-biotechnology.

Sinikeza amamodeli ahlukene amathangi e-electrophoresis avundlile okusakaza nokusebenza amancanei-agaroseama-gels amakhului-agaroseamajeli

Umhlahlandlela Wokukhetha we-Horizontal Immersed Gel Electrophoresis Unit

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekile ekukhiqizeni amathuluzi e-electrophoresis iminyaka engaphezu kwengu-50 neqembu lethu lezobuchwepheshe eliqeqeshiwe kanye nesikhungo se-R&D. Sinomugqa wokukhiqiza onokwethenjelwa nophelele kusukela ekuklanyweni kuya ekuhlolweni, kanye nendawo yokugcina impahla, kanye nokwesekwa kokumaketha. Imikhiqizo yethu eyinhloko i-Electrophoresis Cell (ithangi/igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njll. Siphinde sinikeze amathuluzi aselabhu njenge-PCR instrument, i-vortex mixer kanye ne-centrifuge yaselabhorethri.

Uma unecebo lokuthenga lemikhiqizo yethu, sicela ungangabazi ukusithinta. Ungasithumela umlayezo nge-imeyili[i-imeyili ivikelwe]noma[i-imeyili ivikelwe], noma sicela usishayele ku- +86 15810650221 noma wengeze uWhatsapp +86 15810650221, noma Wechat: 15810650221.

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