I-Polymerase Chain Reaction (PCR) kanye ne-Gel Electrophoresis: Amasu Abalulekile ku-Molecular Biology

Emkhakheni ohlale uvela webhayoloji yamangqamuzana, i-Polymerase Chain Reaction (PCR) kanye ne-Gel Electrophoresis kuye kwavela njengamasu ayisisekelo enza kube lula ukucwaninga nokukhohlisa i-DNA. Lezi zindlela azihlanganisi nje kuphela ucwaningo kodwa futhi zinezinhlelo zokusebenza ezisabalele ekuxilongweni, kwisayensi yezobunhloli, kanye ne-biotechnology.

Isithombe esivela kuwebhusayithi ye-biology4alevel

I-PCR iwuhlelo loguquko olwakhiwa u-Kary Mullis ngo-1983, oluvumela ososayensi ukuthi bakhulise ingxenye ethile ye-DNA ngokugqama. Inqubo iqala nge-denaturation ye-DNA, lapho i-DNA enemicu ekabili ishiselwa cishe ku-94°C, okuyenza ihlukane ibe imicu emibili eyodwa. Lokhu kulandelwa i-annealing, lapho ama-primers—ukulandelana okufushane kwama-nucleotide—kubopha ukulandelana okuhambisanayo ku-DNA enomucu owodwa ezingeni lokushisa eliphansi (imvamisa liba ngu-55°C). Ekugcineni, isigaba sokunwetshwa senzeka ku-72 ° C, lapho i-enzyme i-DNA polymerase ihlanganisa umucu omusha we-DNA ngokwengeza ama-nucleotide kuma-primers. Lo mjikelezo uphindwa izikhathi ezingu-20-40, okuholela ezigidini zamakhophi okulandelana kwe-DNA okuhlosiwe.

Beijing LIUYI PCR Machine

Uma i-DNA isithuthukisiwe, i-Gel Electrophoresis iyasetshenziswa ukuze ihlukanise futhi ihlaziye imikhiqizo ye-PCR. Le nqubo ihilela ukufuduka kwezingcezu ze-DNA ngokusebenzisa i-matrix yejeli ye-agarose ngaphansi kwethonya lenkambu kagesi. Ama-molecule e-DNA akhokhiswa kabi ngenxa yomgogodla wawo we-phosphate, futhi athuthela ku-electrode eqondile. Ijeli isebenza njengesisefo, ivumela izingcezu ze-DNA ezincane ukuthi zihambe ngokushesha kunezinkulu. Ngenxa yalokho, izingcezu ze-DNA ziyahlukaniswa ngokusekelwe kusayizi wazo, ngamabhande ahlukile abonakala ngaphansi kokukhanya kwe-ultraviolet ngemva kokungcoliswa ngodayi ofana ne-ethidium bromide.

Beijing LIUYIImikhiqizo ye-Gel Electrophoresis

Inhlanganisela ye-PCR ne-Gel Electrophoresis inamandla ezinhlelweni eziningi. Ekuxilongweni kwezokwelapha, i-PCR isetshenziselwa ukuthola ubukhona bamagciwane, ukuguqulwa kofuzo, noma ukulandelana okuqondile kwe-DNA okuhlobene nesifo. I-Gel Electrophoresis bese ivumela ukubonwa kanye nokuqinisekiswa kwalezi zingcezu ze-DNA ezikhulisiwe. Kusayensi yezobunhloli, lezi zindlela zibalulekile ekunyathelisweni kweminwe ku-DNA, lapho zisiza khona ukufanisa amasampula e-DNA avela ezigcawini zobugebengu nalawo abasolwa.

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekile ekukhiqizeni amathuluzi e-electrophoresis iminyaka engaphezu kwengu-50 neqembu lethu lezobuchwepheshe eliqeqeshiwe kanye nesikhungo se-R&D. Sinomugqa wokukhiqiza onokwethenjelwa nophelele kusukela ekuklanyweni kuya ekuhlolweni, kanye nendawo yokugcina impahla, kanye nokwesekwa kokumaketha. Imikhiqizo yethu eyinhloko i-Electrophoresis Cell (ithangi/igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njll. Siphinde sinikeze amathuluzi aselabhu njenge-PCR instrument, i-vortex mixer kanye ne-centrifuge yaselabhorethri.

Beijing LIUYIImikhiqizo ye-Gel Electrophoresis

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