I-Polyacrylamide Gel Electrophoresis

I-Polyacrylamide ivamise ukusetshenziswa ekusetshenzisweni kwebhayoloji yamangqamuzana njengendlela ye-electrophoresis yamaprotheni nama-nucleic acid ngendlela eyaziwa ngokuthi IKHASI. Kuwuhlobo lwe-zone electrophoresis indlela ngejeli yokwenziwa ebizwa nge-polyacrylamide njengendlela esekelayo. Yakhiwa ngu-S.Raymond kanye no-L.Weintraub ngo-1959, yase ithuthukiswa futhi yathuthukiswa ngu-L.Ornstein kanye no-BJ Davis. Le ndlela yasetshenziswa kabanzi ngemva kokuchazwa okwengeziwe nokuguqulwa yibo kuthiyori kanye namasu okuhlola ngo-1964.

Ngaphambi kokufaka i-polyacrylamide gel electrophoresis, abantu ikakhulukazi basebenzisa i-electrophoresis yephepha ye-zone EP. Kepha iphepha lisebenza njenge-medium, libe nomsebenzi we-anti-convection, awukho omunye umphumela omuhle. Ngenkathi ijeli ye-polyacrylamide ingenawo umsebenzi we-anti-convection kuphela kodwa futhi ingabamba iqhaza ekuqhubekeni kokuhlukaniswa ngenkuthalo. Ngoba ijeli ye-polyacrylamide iwuhlobo lwesakhiwo senetha, okuyinhlanganisela ye-polymerization ne-cross-linking ye-acrylamide (Acr) ne-N,N-methylenebis(acrylamide). I-Acrylamide ibizwa nge-monomer, kuyilapho i-N,N-methylenebis ibizwa nge-comonomer noma i-crosslinker. Ukwakhiwa kwejeli kuyinqubo ye-polymerization yamakhemikhali. Ubukhulu bembotshana yejeli bungalawulwa, ngaleyo ndlela ijeli ingenziwa ngamadigri ahlukene okuxhumanisa. Uma usayizi wezimbotshana usondela ku-radius emaphakathi ye-molecule yesampula, ukumelana kwe-molecule ukuthi idlule embotsheni yejeli kuzoba nobudlelwane obuseduze nosayizi nokuma kwe-molecule ngesikhathi se-electrophoresis. Ngakho-ke inikeza isici sokuhlukanisa esishintshayo sokuhlukanisa lezo zinto ezinezindleko ezifanayo.

Kunezindlela ezimbili ezijwayelekile ezisetshenziselwa i-polyacrylamide gel electrophoresis, eyodwa i-disc electrophoresis, kanti enye i-slab electrophoresis. I-slab electrophoresis yakhelwe ngokukhethekile ukuhlukanisa amaprotheni ne-DNA, futhi ngokuvamile, kunezinhlobo ezimbili ze-slab electrohporesis, okuyithangi le-electrophoresis elivundlile kanye nethangi le-electrophoresis eliqondile. Kumaprotheni, abantu basebenzisa ithangi le-electrophoresis elivundlile le-IFF kanye ne-immunoelectrophoresis, ngaphandle kwalokho, abantu basebenzisa amathangi e-electrophoresis aqondile amaprotheni.

I-Beijing Liuyi Biotechnology inezinhlobo ezahlukene zamathangi e-slab electrophoresis eKHASI, ngaphandle kokuhlaziywa nokuhlonza amasampula amaprotheni nge-polyacrylamide gel electrophoresis, ingasetshenziswa futhi ukukala isisindo samangqamuzana amasampula, ukuhlanza amasampula nokulungiselela amasampula.

Thatha imodeliI-DYCZ-23Anjengesibonelo, okuyithangi le-electrophoresis elijwayelekile lelebhu. Sebenzisa amapuleti engilazi amabili ukwakha igumbi lejeli ukuze wenze ijeli, bese ushaya amapuleti engilazi ngokuqinile ukuze uvimbele ukuvuza kwejeli. Ubukhulu bejeli buncike ekugqineni kwesikhala. Imvamisa, ubukhulu bungu-1.5mm ukuze kukhishwe ukushisa okungcono, futhiI-DYCZ-23Afuthi inikeza izikhala zokujiya ezingu-1.0mm zokusakaza ijeli engu-1.0mm. Ngaphandle kwethangi le-electrophoresis, ukuze usebenzise i-electrophoresis, ukunikezwa kwamandla nakho kuyadingeka. I-Beijing Liuyi Biotechnology inikeza izinhlobonhlobo zeukunikezwa kwamandla kwe-electrophoresis. Ukusuka ku-voltage ephezulu kuye ku-voltage ephansi, ukhetha imodeli ngokuya ngohlelo lokusebenza.

I-Beijing Liuyi brand inomlando weminyaka engaphezu kwengama-50 e-China futhi inkampani ingahlinzeka ngemikhiqizo ezinzile nesezingeni eliphezulu emhlabeni jikelele. Ngeminyaka yokuthuthuka, kufanelekile ukukhetha kwakho!

Manje sifuna ozakwethu, kokubili ithangi le-OEM electrophoresis kanye nabasabalalisi bamukelekile.

Ukuze uthole ukwaziswa okwengeziwe ngathi, sicela usithinte nge-imeyili[i-imeyili ivikelwe]noma[i-imeyili ivikelwe].