Isingeniso
I-Gel electrophoresis iyindlela eyisisekelo kubhayoloji yamangqamuzana, esetshenziswa kabanzi ekuhlukaniseni amaprotheni, ama-nucleic acid, namanye ama-macromolecules. Ukulawulwa okufanele kwevolumu yesampula, i-voltage, nesikhathi se-electrophoresis kubalulekile ukuze kuzuzwe imiphumela enembile nekwazi ukukhiqizwa kabusha.Uzakwethu wethu welebhu unikezaimikhuba engcono kakhulu yokuphatha le mingcele phakathi ne-SDS-PAGE gel electrophoresis.
Imikhiqizo yaseBeijing Liuyi Biotechnology gel electrophoresis
Umthamo Wesampula: Ukuqinisekisa Ukuvumelana
Uma wenza i-SDS-PAGE electrophoresis, umthamo wesampula uyisici esibalulekile esingaba nomthelela omkhulu ekuxazululeni kwemiphumela yakho. Ngokuvamile kunconywa ukulayisha u-10 µL wengqikithi yamaprotheni emthonjeni ngamunye. Ukuqinisekisa ukungaguquguquki nokuvimbela ukuhlukaniswa kwesampula phakathi kwemithombo eseduze, kubalulekile ukulayisha ivolumu elinganayo ye-1x yokulayisha ibuffer kunoma yimiphi imithombo engenalutho. Lokhu kuqapha kusiza ukuvimbela ukusabalala kwamasampula emigwaqweni engumakhelwane, okungenzeka uma umthombo ushiywe ungenalutho.
Ngaphambi kokulayisha amasampula akho, njalo qala ngokungeza umaka wesisindo semolekyuli emthonjeni owodwa. Lokhu kuvumela ukuhlonza kalula osayizi bamaprotheni ngemuva kwe-electrophoresis.
Ukulawula I-Voltage: Isivinini Sokulinganisa Nokulungiswa
I-voltage esetshenziswa ngesikhathi se-electrophoresis ithinta ngokuqondile kokubili isivinini amasampula ahamba ngaso ngejeli kanye nesinqumo sokuhlukaniswa. Ku-SDS-PAGE, kuyatuseka ukuthi uqale nge-voltage ephansi cishe engu-80V. Le voltage yokuqala ephansi ivumela amasampula ukuthi ahambe kancane futhi ngokulinganayo, awagxilise ebhandeni elibukhali njengoba engena ejeli ehlukanisayo.
Uma amasampula esengene ngokugcwele ejeli ehlukanisayo, i-voltage inganyuswa ibe ngu-120V. La mandla kagesi aphezulu asheshisa ukufuduka, aqinisekise ukuthi amaprotheni ahlukaniswa kahle ngokwesisindo samangqamuzana awo. Kubalulekile ukuqapha ukuqhubeka kwe-bromophenol blue dye front, ekhombisa ukuqedwa kwe-electrophoresis. Kuma-gel ane-concentration ye-10-12%, imizuzu engu-80-90 ngokuvamile yanele; kodwa-ke, kuma-gel angu-15%, kungase kudingeke wengeze isikhathi sokugijima kancane.
Ukuphatha Isikhathi: Ukwazi Isikhathi Sokumisa
Isikhathi singesinye isici esibalulekile ku-gel electrophoresis. Ukusebenzisa ijeli isikhathi eside kakhulu noma esifushane kakhulu kungaholela ekuhlukaneni okungafanele. Ukufuduka kwedayi ye-bromophenol eluhlaza okwesibhakabhaka kuyinkomba ewusizo: uma ifika phansi kwejeli, ngokuvamile kuyisikhathi sokumisa ukugijima. Kumajeli ajwayelekile, afana no-10-12%, ubude besikhathi se-electrophoresis cishe imizuzu engama-80-90 buvame ukwanele. Kumaphesenti aphezulu ama-gel, afana ne-15%, isikhathi sokugijima kufanele sinwetshwe ukuze kuqinisekiswe ukuhlukaniswa okuphelele kwamaprotheni.
Ukuphathwa Kwesibhafa: Ukusebenzisa Kabusha Nokulungiselela Amabhafa
I-Electrophoresis buffer ingasetshenziswa kabusha izikhathi ezi-1-2, kuye ngezimo ezithile zelabhorethri yakho. Kodwa-ke, ukuze uthole imiphumela emihle, kuyanconywa ukuthi ulungise i-buffer entsha engu-10x futhi uyihlambulule ngaphambi nje kokuyisebenzisa. Lokhu kuqinisekisa ukuthi i-buffer igcina ukusebenza kwayo, okuholela emiphumeleni ethembeke kakhudlwana ye-electrophoresis.
Imikhiqizo yaseBeijing Liuyi Biotechnology gel electrophoresis
Ngokulawula ngokucophelela umthamo wesampula, i-voltage, nesikhathi se-electrophoresis, ungathuthukisa ngokuphawulekayo ukunemba nokukhiqizwa kabusha kwemiphumela ye-gel electrophoresis yakho. Ukusebenzisa lezi zinqubo ezihamba phambili emsebenzini wakho waselabhorethri kuzokusiza ufinyelele amabhendi acacile nahluke kakhulu, okuholela kudatha engcono yokuhlaziya umfula.
Uma unezindlela eziningi ezinhle zokuthuthukisa ukuhlolwa kwe-gel electrophoresis, wamukelekile ukuxoxa nathi!
I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekile ekukhiqizeni amathuluzi e-electrophoresis iminyaka engaphezu kwengu-50 neqembu lethu lezobuchwepheshe eliqeqeshiwe kanye nesikhungo se-R&D. Sinomugqa wokukhiqiza onokwethenjelwa nophelele kusukela ekuklanyweni kuya ekuhlolweni, kanye nendawo yokugcina impahla, kanye nokwesekwa kokumaketha. Imikhiqizo yethu eyinhloko i-Electrophoresis Cell (ithangi/igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njll. Siphinde sinikeze amathuluzi aselabhu njenge-PCR instrument, i-vortex mixer kanye ne-centrifuge yaselabhorethri.
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Isikhathi sokuthumela: Aug-20-2024