Lapho kwenziwa ukuhlaziya okuqhathanisayo kwemiphumela ye-electrophoresis, izici ezimbalwa zingaholela ekwehlukeni kwedatha:
Ukulungiselela Isampula:Ukuhlukahluka kokugxilwa kwesampula, ukuhlanzeka, nokuwohloka kungathinta imiphumela ye-electrophoresis. Ukungcola noma i-DNA/RNA eyonakele kusampula kungabangela ukugcotshwa noma amabhande angacacisiwe.
Ukugxilwa kweGel nohlobo:Ukugxila kanye nohlobo lwejeli (isb, i-agarose noma i-polyacrylamide) kuthinta ukulungiswa kokuhlukaniswa kwamangqamuzana. Amajeli okugxilisa aphakeme angcono ukuhlukanisa ama-molecule amancane, kuyilapho amajeli okugxilisa aphansi afanele ama-molecule amakhulu.
Izimo ze-Electrophoresis:Amandla enkundla kagesi (i-voltage), isikhathi se-electrophoresis, kanye nohlobo ne-pH ye-buffer esebenzayo konke kungathinta imiphumela. I-voltage ephezulu kakhulu ingabangela umsila webhande noma ukulungiswa okuncishisiwe, futhi isikhathi se-electrophoresis esandisiwe singaholela ekusakazweni kwebhendi.
Ikhwalithi nokulungiselela kwe-Buffer:Amabhafa angalungile noma aphelelwe yisikhathi angaholela ekushintsheni ku-pH namandla e-ionic, athinte ukuhamba kwamangqamuzana nokulungiswa.
Inani Lokulayisha Isampula kanye Nobuchule:Ukulayisha ngokweqile noma ukulayisha ngaphansi kwamasampula kungathinta ukucaca kwebhendi nokuqina. Ukulayisha okungalingani kungase kuholele ekusabalaleni kwesampula noma emizileni egwegwile.
Izisetshenziswa ze-Electrophoresis kanye Nezimo Zemvelo: Imishini ye-electrophoresis ehlukene (njengamathangi ejeli nezinto zikagesi) nezimo zemvelo (njengezinga lokushisa nomswakama) zingathinta ukuzinza nokukhiqiza kabusha kwemiphumela ye-electrophoresis.
Izindlela zokufaka amabala kanye nokubona:Ukukhethwa kwebala (isb., i-ethidium bromide, i-SYBR Green) kanye nesikhathi sokungcolisa kungathinta ukucaca nokubonwa kwamabhendi.
Ikhwalithi ye-Electrophoresis Gel:Amabhamuza kumajeli enziwe ekhaya, ikhwalithi yejeli engalingani, noma amajeli awonakele angabangela amabhande ukuthi agobe noma afuduke ngendlela engavamile.
Isakhiwo kanye nosayizi we-DNA/RNA:Ukuthi i-DNA noma i-RNA kusampula iwumugqa, iyindilinga, noma ihlanganiswe kakhulu, noma usayizi wezingcezu, kuzothinta isivinini sazo sokufuduka kujeli.
Umlando Wokuphatha Isampula:Izinto ezifana nenani lemijikelezo yokuncibilika kweqhwa, izinga lokushisa lokugcina, nobude besikhathi kungathinta ubuqotho besampula, ngaleyo ndlela kube nomthelela emiphumeleni ye-electrophoresis.
Uchwepheshe we-Liuyi Biotechnology wenza ucwaningo lwe-electrophoresis elebhu
Siyakwamukelaukuze uxhumane nathi ukuze sixoxe ngezinto ezingabangela umehluko kudatha ye-electrophoresis. Ngokuqonda lezi zici, singanciphisa ukuhlukahluka kudatha, sithuthukise ukuphindaphindeka kokuhlolwa kanye nokunemba kwemiphumela..
I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekile ekukhiqizeni amathuluzi e-electrophoresis iminyaka engaphezu kwengu-50 neqembu lethu lezobuchwepheshe eliqeqeshiwe kanye nesikhungo se-R&D. Sinomugqa wokukhiqiza onokwethenjelwa nophelele kusukela ekuklanyweni kuya ekuhlolweni, kanye nendawo yokugcina impahla, kanye nokwesekwa kokumaketha. Imikhiqizo yethu eyinhloko i-Electrophoresis Cell (ithangi/igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njll. Siphinde sinikeze amathuluzi aselabhu njenge-PCR instrument, i-vortex mixer kanye ne-centrifuge yaselabhorethri.
Uma unecebo lokuthenga lemikhiqizo yethu, sicela ungangabazi ukusithinta. Ungasithumela umlayezo nge-imeyili[i-imeyili ivikelwe]noma[i-imeyili ivikelwe], noma sicela usishayele ku- +86 15810650221 noma wengeze uWhatsapp +86 15810650221, noma Wechat: 15810650221.
Sicela Skena ikhodi ye-QR ozoyengeza kuWhatsapp noma ku-WeChat.
Isikhathi sokuthumela: Sep-12-2024