Amaseli ezinto eziphilayo akhiwa izinhlobonhlobo zamangqamuzana amakhulu namancane. Ukuqonda ukwakheka nokusebenza kwamangqamuzana ezinto eziphilayo kuyisisekelo sokuhlolisisa izimfihlo zokuphila.
Amangqamuzana amancane ebhayoloji ngokuvamile ahlukaniswa ngezigaba ezinkulu eziningana, njengama-carbohydrate, ama-lipids, ama-amino acid, ama-nucleotide, nokunye. Ukubaluleka kwala mamolekyuli amancane ezinto eziphilayo akukhona nje endimeni yawo njengezisekelo zokwakha ama-macromolecules amakhulu ezinto eziphilayo, njengamaprotheni akhiwa ama-amino acid, ama-nucleic acid akhiwa ama-nucleotides, nama-polysaccharides akhiwa ama-monosaccharides, kodwa naseqinisweni lokuthi amaningi kulawa ma-molecule amancane ngokwawo adlala imisebenzi ebalulekile yomzimba ngaphakathi kwamaseli.
Amaprotheni, ama-nucleic acid, nama-polysaccharides yizigaba ezintathu ezinkulu zama-macromolecules ephilayo.
Ubuchwepheshe be-Electrophoresis bungasetshenziswa ukuhlaziya nokunquma ubukhulu bama-molecule ahlukahlukene, angagcini nje kuma-nucleic acid kodwa ahlanganisa amaprotheni, ama-peptide, ama-molecule amancane, nokunye. Ukukhetha indlela efanele ye-electrophoresis nezimo kwenziwa ngokusekelwe esisindweni samangqamuzana ofuna ukuwahlaziya.
Nazi ezinye izincomo ezijwayelekile zokukusiza ukhethe indlela efanele ye-electrophoresis esekelwe esisindweni samangqamuzana:
Amamolekyuli Amakhulu: Uma udinga ukuhlaziya ama-molecule amakhulu njengezingcezu ezinkulu ze-DNA noma amaprotheni, ngokuvamile usebenzisa izindlela ze-electrophoresis zejeli njenge-agarose gel electrophoresis noma i-polyacrylamide gel electrophoresis. Lawa ma-gel angakwazi ukuhlalisa ama-molecule amakhulu futhi awahlukanise ngokuphumelelayo.
I-Electrophoresis TankI-DYCP-31DNi-agarose gel electrophoresis
Amamolekyuli anosayizi omaphakathi: Ama-molecule anosayizi omaphakathi afana nezingcezu ze-DNA zamakhulu ambalwa amapheya ayisisekelo noma amaprotheni kuhla lwezinkulungwane ezimbalwa kuya emashumini ezinkulungwane zamaDaltons angahlukaniswa kusetshenziswa i-polyacrylamide gel electrophoresis, okuyindlela evame ukusetshenziswa kubantu abaningi abaphakathi nendawo. ama-molecule amancane.
I-Electrophoresis Tank I-DYCZ-24DN amaprothenii-gel electrophoresis
Amangqamuzana Amancane: Kuma-molecule amancane afana namaprotheni angaphansi kwamakhulu ambalwa ama-Daltons, ama-peptides, noma amakhompiyutha amancane, amasu okuxazulula okuphezulu njenge-capillary electrophoresis noma i-capillary gel electrophoresis angasetshenziswa. Lezi zindlela zizwela kakhulu ekuhlukaniseni nasekutholeni ama-molecule amancane.
Ama-molecule Amakhulu Kakhulu: Uma udinga ukuhlaziya ama-molecule amakhulu kakhulu, njenge-DNA enkulu ye-genomic, ngokuvamile usebenzisa amasu afana ne-pulsed-field gel electrophoresis (PFGE) noma izindlela ezikhethekile eziklanyelwe ukuphatha ama-molecule amakhulu kangaka.
I-Electrophoresis enezinhlangothi ezimbili: Ngezingxube eziyinkimbinkimbi noma lapho udinga ukuhlaziya ama-molecule anosayizi abahlukene, i-electrophoresis enezinhlangothi ezimbili ingaba yisinqumo esihle. Le ndlela ihlanganisa amasu amabili noma ngaphezulu e-electrophoresis ukuze kuzuzwe ukulungiswa okuphezulu.
2-D Amaprotheni Electrophoresis CellI-DYCZ-26C
I-Beijing Liuyi Biotechnology Co., Ltd. (eyayiyi-Beijing Liuyi Instrument Factory) yasungulwa ngo-1970. Ibhizinisi lobuchwepheshe eliphethwe nguhulumeni eligxile ekukhiqizeni amathuluzi okuhlaziya elabhorethri yamangqamuzana ezinto eziphilayo. Le nkampani inenhlokodolobha ebhalisiwe yama-yuan ayizigidi ezingama-20. Inabasebenzi abangama-80 sebebonke.
U-Liuyi uye wasebenza ngokukhethekile ekukhiqizeni amathuluzi e-electrophoresis iminyaka engaphezu kwengu-50 ngeyayoithimba lezobuchwepheshe elihambayo kanye nesikhungo se-R&D.Ithas umugqa wokukhiqiza onokwethenjelwa nophelele kusukela ekuklanyweni kuya ekuhlolweni, kanye nendawo yokugcina impahla, kanye nokwesekwa kokumaketha.Iimikhiqizo eyinhloko i-Electrophoresis Cell (ithangi/igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, iGel Image & Analysis System njll.
Ihlinzeka ngemikhiqizo ehlukahlukene ye-eletrophoresishlaziyaingfuthi unqumeingubukhulu bama-molecule ahlukahlukene. Khetha i-Beijing Liuyi Biotechnology futhi uyivumele ikusize wenze isilingo.
Manje sifuna ozakwethu, kokubili ithangi le-OEM electrophoresis kanye nabasabalalisi bamukelekile.
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Isikhathi sokuthumela: Oct-24-2023