Yini i-DNAukulandelana?
Kuyinqubo yokunqumaukulandelana okuqondile noma ukuhleleka kwezisekelo (A, C, G kanye no-T) ku-molecule ye-DNA.Kungani kudingeka sazi ukulandelana kwe-DNA ezakhini zofuzo ezithile? Lapha sazi ezinye izinhlelo zokusebenza. Okokuqala, ingasisiza ukuba sibone ukuguquka kwezakhi zofuzo ezithile. Khona-ke uma sazi ukuhleleka kokulandelana kwe-DNA, kuyosisiza ukuba sikwazi ukuhlukanisa into ephilayo kwenye into ephilayo.Kungasisiza futhi ukuba sibone umuntuama-haplotypeskanye dukuhlukanisa ama-polymorphisms.
Kubaluleke ngempela futhi kuwusizo ukucwaninga amasu okulandelana kwe-DNA. E-Beijing Liuyi Biotechnology Co., Ltd, sinikeza i-DYCZ-20 uchungechunge lwe-electrophoresis apparatus ukusiza abacwaningi ukuthi bahlole ukulandelana kwe-DNA, ukuhlaziywa kwe-DNA yeminwe, ukubonisa okuhlukile njll.
Muva nje, uchwepheshe kulebhu yethu uzobonisa ukuthi kwenziwa kanjani ijeli nge-DYCZ-20F electrophoresis apparatus yethu. Uchwepheshe uMnu.Wu wabelane ngezindlela zokujova ijeli egumbini lengilazi.
I-DYCZ-20F electrophoresis apparatus ingasakaza usayizi wejeli ongu-340x450mm. Okokuqala, sidinga ukulungiselela wonke umsebenzi esiwudingayo ukuze siphonse ijeli, njengesixazululo sejeli, gipuleti lokugcina,gel ibhodlela, ushaka amazinyo kama, i-spacer(0.4mm), iziqeshana.
Ngaphambi kokuphonsa ijeli, sidinga ukuhlanza futhi silungise ipuleti yengilazi kuqala. Lapha, umhloli wancoma ukusebenzisa amanzi ukuhlanza ingaphezulu lamapuleti engilazi. Bese ugcoba i-Bind -silane phezu kwepuleti lengilazi ukusiza ijeli ukuthi inamathele epuleti lengilazi, bese wembathisa i-Repel-silaneokuphawuliwe ipuleti lengilazi ukuze ukhiphe ijeli kalula.
Umhloli ubonise izindlela ezimbili zokujova isisombululo sejeli:
Tshekisa kancane igumbi lengilazi yejeli, bese ujova ikhambi lejeli ngokulinganayo emuva naphambili. (Amabhamuza omoya kufanele agwenywe ukukhiqiza phakathi nale nqubo. )
Uma umuntu ohlolayo ekhetha ngale ndlela, kufanele azilolonge kaningi ukuze aphumelele ngale ndlela.
Kwabaqalayo, sincoma le ndlela yokwenza ijeli. Shaya izinhlangothi ezintathu ngeziqeshana , bese utshekisa igumbi lengilazi yejeli nge-engeli engu-45 degree, bese ujova ikhambi lejeli ngakwesokunene sakho kuze kugcwale ikhambi lejeli kulo lonke igumbi lejeli.
Ngifisa sengathi lokhu kuboniswa kwejeli yokusakaza kungasiza ikhasimende lethu ukuthi lilungiselele ukuhlolwa kwalo.
Ithuluzi le-Beijing Liuyi DYCZ-20 le-DNA yokuhlaziya ukulandelana kwe-electrophoresis lisetshenziselwa ukutholwa kokuguqulwa kofuzo. Ukuguqulwa kofuzo kungenye yezimbangela eziyinhloko zezifo eziningi zofuzo. Nge-electrophoresis ehlaziya i-DNA kusampula, ukuguqulwa kwezakhi zofuzo kungatholwa, ngaleyo ndlela kusize odokotela banqume ukuthi isiguli siphethe isakhi sofuzo esibangela isifo. Lolu chungechunge lungaphinda lusetshenziselwe i-genotyping. I-Genotyping ibhekisela ekuhlaziyweni kwe-loci ethile ku-genome yomuntu ukuze kutholakale uhlobo lwe-genotype yabo. Lokhu kubalulekile kubacwaningi ngoba izinhlobo ezahlukene zofuzo zingase zihlotshaniswe nokuba sengozini yezifo ezithile noma ukusabela ezidakamizweni. Ngokusebenzisa ithuluzi le-electrophoresis yokuhlaziya ukulandelana kwe-DNA ye-Liuyi, i-genotyping ingenziwa ngokushesha nangokunembile, inikeze isisekelo esibalulekile somuthi womuntu siqu. Ngaphezu kwalokho, lolu chungechunge lwezinsimbi lungabuye lusetshenziselwe ukuhlela ukulandelana kwe-DNA. Ukulandelana kwe-DNA kuyindlela ebalulekile yokuthi abacwaningi baqonde ukwakheka nokusebenza kwe-genome. Ngokubeka amasampula e-DNA ekuhlaziyweni kwe-electrophoretic, ukuhleleka kwezisekelo ngokulandelana kwe-DNA kunganqunywa, kusize abacwaningi bahumushe ulwazi ku-genome. Siyakwamukela ukuxhumana nathi ukuze uthole ulwazi olwengeziwe lwemikhiqizo.
Manje sifuna ozakwethu, kokubili ithangi le-OEM electrophoresis kanye nabasabalalisi bamukelekile.
Uma unecebo lokuthenga lemikhiqizo yethu, sicela ungangabazi ukusithinta. Ungasithumela umlayezo nge-imeyili[i-imeyili ivikelwe]noma[i-imeyili ivikelwe], noma sicela usishayele ku- +86 15810650221 noma wengeze uWhatsapp +86 15810650221, noma Wechat: 15810650221
Isikhathi sokuthumela: Oct-12-2023