I-Agarose Gel Electrophoresis ye-RNA

Ucwaningo olusha oluvela ku-RNA

Muva nje, ucwaningo luthola ukuthi ukuhlukahluka kofuzoezehlisa amazinga okuhlela e-RNA enemicu ephindwe kabili ahlotshaniswa nezimo ezizimele kanye ne-immune-mediated.

Ama-molecule e-RNA angashintshwa. Isibonelo, ama-nucleotide angase afakwe, asuswe, noma ashintshwe. Enye yezinhlelo ezivame kakhulu, ucwaningo olusha olubonisa ukuthi ludlala indima ebalulekile ekuqaleni kwesifo sokuvuvukala, ukuguqulwa kwe-nucleotide adenosine ibe inosine ngaphakathi kwe-RNA enezintambo ezimbili. Ucwaningokwabaeshicilelweon Agasti 3 kuMvelo wembula ukuthi okuhlukile kofuzo okwehlisa lokhu kuguqulwa okuthile kuhlotshaniswa nengozi eyengeziwe yokuvuvukala kwe-autoimmune kanye ne-immune-mediated inflammatory disorders njenge-psoriasis, isifo samathumbu esivuvukalayo, nesifo sikashukela sohlobo loku-1. Ababhali baphakamisa ukuthi iphrotheni yezinzwa kungenzeka yenza iphutha kulawa ma-RNA angahlelwanga kangako kuma-molecule angaphandle, okubangela impendulo yokuvuvukala.

Isazi samangqamuzana ezinto eziphilayo sacabanga kanjalo'ngempela impumelelo enkulu, futhi etholakele"kuyamangaza ngempela" ukuthi ukuhlelwa kwe-RNA kubonakala kubaluleke kangakanani ezifweni ezivuvukalayo, uma kuqhathaniswa nokuvezwa kofuzo noma ukuhlanganisa.

Kuwucwaningo olukhulu ngempela. Futhi njengokuthengiswa kwemikhiqizo ye-electrophoresis, ngithanda ukwazi ukuthi iyini i-RNA, nokuthi ososayensi bayihlaziya kanjani i-RNA nge-electrophoresis? Kuwulwazi oluchwepheshile kakhulu futhi engingalwazi kimina kodwa noma kunjalo kumnandi ukuluhlola.

Iyini i-RNA?

I-RNA, ifushane nge-Ribonucleic acid, i-nucleic acid ekhona kuwo wonke amangqamuzana aphilayo. Ngokungafani ne-DNA, i-RNA ivame ukuba nomucu owodwa. Indima yayo eyinhloko iwukusebenza njengesithunywa esiphethe imiyalelo evela ku-DNA yokulawula ukuhlanganiswa kwamaprotheni, nakuba kwamanye amagciwane i-RNA kune-DNA iphethe ulwazi lofuzo.

I-Agarose Gel Electrophoresis ye-RNA

Abantu bangakwazihlola ikhwalithi ye-RNA nge-gel electrophoresis.

I-Beijing Liuyi Biotechnology Co.Ltd, i-aUmkhiqizi ochwepheshe ogxile emikhiqizweni ye-electrophoresis ngaphezu kweminyaka engama-50, unikeza amathangi e-electrophoresis (amagumbi/amaseli) e-gel electrophoresis, njengamathangi e-electrophoresis avundlile (amakamelo/amaseli) e-agarose gel electrophoresis, namathangi e-electrophoresis aqondile (amakamelo/amaseli) i-protein gel electrophoresis. Phakathi naleso sikhathi, ihlinzeka ngezinhlobo ezahlukene zokunikezwa kwamandla kagesi we-electrophoresis, i-cabinet dark box analyzer kanye ne-UV transilluminator. Ungakhetha Beijing Liuyi's amathangi e-electrophoresis (amagumbi/amaseli) ukukala isisindo se-RNA kanye nokuhlukanisa i-RNA yosayizi abahlukene ngelebhu yakho.

Lapha sizokwethula uhlobo olulodwa lwethangi le-electrophoresis elivundlile (igumbi/iseli) imodeli ye-DYCP-31DN emikhiqizweni yethu yochungechunge engama-31.

I-DYCP-31DN ithangi le-electrophoresis likasayizi omncane ovundlile(igumbi/iseli) lokukala isisindo semolekyuli ye-RNA noma i-DNA nokuzehlukanisa.