Ubukhulu (LxWxH) | 433×320×308mm |
Isibani | Isibani se-DC12V 22W se-Tungsten halogen |
Indlela ye-Optical | Isiteshi sendlela yokukhanya eqondile engu-8 |
Ububanzi be-wavelength | 400-900nm |
Hlunga | Ukucushwa okuzenzakalelayo 405, 450, 492, 630nm, kungafakwa kufikela kuzihlungi eziyi-10. |
Ibanga lokufunda | 0-4.000Abs |
Isixazululo | 0.001Abs |
Ukunemba | ≤±0.01Abs |
Ukuzinza | ≤±0.003Abs |
Ukuphindaphinda | ≤0.3% |
Ipuleti lokudlidliza | Izinhlobo ezintathu ze-linear vibration plate function, imizuzwana engu-0-255 elungisekayo |
Bonisa | Isikrini se-LCD esingu-8 intshi, sibonise lonke ulwazi lwebhodi, ukusebenza kwesikrini sokuthinta |
Isofthiwe | Isoftware yobungcweti, ingagcina uhlelo lwamaqembu ayi-100, imiphumela eyisampula eyi-100000, izinhlobo ezingaphezu kweziyi-10 zezibalo zokulinganisa ijika |
Okokufaka kwamandla | I-AC100-240V 50-60Hz |
I-Mircoplate Reader ingasetshenziswa kakhulu ezindaweni zokucwaninga, amahhovisi okuhlola ikhwalithi kanye nezinye izindawo zokuhlola ezifana nezolimo nokufuywa kwezilwane, amabhizinisi okuphakelayo kanye nezinkampani zokudla. Imikhiqizo akuzona ezokwelashwa, ngakho-ke ngeke ithengiswe njengemishini yezokwelapha noma isetshenziswe ezikhungweni zezokwelapha ezifanele.
• Isibonisi se-LCD sombala wezinga lezimboni, ukusebenza kwesikrini sokuthinta.
• Isistimu yokulinganisa i-fiber optical yamashaneli ayisishiyagalombili, umtshina ongenisiwe.
• Umsebenzi wokumisa phakathi nendawo, unembile futhi unokwethenjelwa.
• Izinhlobo ezintathu zomsebenzi wepuleti lokudlidliza elingumugqa.
• Ifomula yokwahlulela evulekile evulekile yokusika,Cabanga ukuthi ucabangani.
• Indlela yephoyinti lokugcina, indlela yamaphuzu amabili, amandla, imodi yokuhlola eyodwa/embili yamaza.
• Lungiselela imojula yokulinganisa izinga lokuvinjelwa, elinikezelwe emkhakheni wokuphepha kokudla.
1.Iyini i-microplate reader?
Isifundi se-microplate ithuluzi laselabhorethri elisetshenziselwa ukuthola kanye nenani lezinqubo zebhayoloji, amakhemikhali, noma zomzimba kumasampuli aqukethwe ngaphakathi kwama-microplates (abuye aziwe ngokuthi amapuleti ama-microtiter). Lawa mapuleti ngokuvamile akhiwa imigqa namakholomu emithombo, ngalinye likwazi ukubamba umthamo omncane woketshezi.
2.Yini engakala umfundi we-microplate?
Abafundi be-Microplate bangakwazi ukukala imingcele eminingi, kuhlanganise nokumunca, i-fluorescence, i-luminescence, nokuningi. Izicelo ezijwayelekile zifaka ukuhlolwa kwe-enzyme, izifundo zokusebenza kwamaseli, i-protein ne-nucleic acid quantification, ama-immunoassays, nokuhlolwa kwezidakamizwa.
3.Isebenza kanjani i-microplate reader?
Isifundi se-microplate sikhipha ubude beza obuthile bokukhanya emithonjeni yesampula futhi sikala amasignali angumphumela. Ukusebenzisana kokukhanya namasampuli kunikeza ulwazi mayelana nezakhiwo zawo, njengokumunca (okwezinhlanganisela ezinemibala), i-fluorescence (yezinhlanganisela ze-fluorescent), noma i-luminescence (ngokusabela okukhipha ukukhanya).
4.Iyini i-absorbence, i-fluorescence, ne-luminescence?
I-Absorbance: Lokhu kulinganisa inani lokukhanya elimuncwa isampula kubude obuthile begagasi. Ngokuvamile isetshenziselwa ukulinganisa ukuhlangana kwezinhlanganisela ezinemibala noma umsebenzi wama-enzyme.
I-Fluorescence: Ama-athomu e-Fluorescent amunca ukukhanya kubude obubodwa be-wavelength futhi akhiphe ukukhanya ngobude obude begagasi. Lesi sakhiwo sisetshenziselwa ukutadisha ukusebenzisana kwamangqamuzana, ukubonakaliswa kofuzo, nezinqubo zamaselula.
I-Luminescence: Lokhu kulinganisa ukukhanya okukhishwa kusampula ngenxa yokusabela kwamakhemikhali, njenge-bioluminescence kusukela ekuphenduleni kwe-enzyme-catalyzed. Ivamise ukusetshenziselwa ukutadisha imicimbi yeselula ngesikhathi sangempela.
5.Kuyini ukubaluleka kwezindlela ezihlukene zokubona?
Ukuhlolwa okuhlukile nokuhlola kudinga izindlela zokutholwa ezithile. Isibonelo, ukumunca kuyasiza ekuhlolweni kwe-colorimetric, kuyilapho i-fluorescence ibalulekile ekufundeni ama-biomolecules ane-fluorophores, futhi i-luminescence isetshenziselwa ukutadisha izenzakalo zamaselula ezimweni zokukhanya okuphansi.
6.Ihlaziywa kanjani imiphumela yokufunda kwe-microplate?
Abafundi beMicroplate bavame ukuza nesoftware ehambisana nayo evumela abasebenzisi ukuthi bahlaziye idatha eqoqiwe. Le softhiwe isiza ukulinganisa imingcele elinganisiwe, idale amajika ajwayelekile, futhi ikhiqize amagrafu ukuze ichazwe.
7.Iyini ijika elijwayelekile?
Ijika elijwayelekile liwumfanekiso wesithombe sokugxila okwaziwayo kwento esetshenziselwa ukuhlobanisa isiginali ekhiqizwe yisifundi se-microplate nokugxiliswa kwento kusampula engaziwa. Lokhu kuvame ukusetshenziswa ekuhlolweni kwe-quantification.
8.Ngingakwazi ukwenza izilinganiso ngokuzenzakalelayo ngesifundi se-microplate?
Yebo, abafundi be-microplate bavame ukuhlonyiswa ngezici ezizenzakalelayo ezikuvumela ukuthi ulayishe amapuleti amaningi futhi uhlele izilinganiso ngezikhathi ezithile zesikhathi. Lokhu kuwusizo ikakhulukazi ekuhloleni komkhiqizo ophezulu.
9. Yiziphi izinto ezibalulekile okufanele uzicabangele lapho usebenzisa i-microplate reader?
Cabangela izici ezifana nohlobo lokuhlolwa, imodi yokuthola efanele, ukulinganisa, ukusebenzisana kwepuleti, nokulawulwa kwekhwalithi yama-reagents asetshenzisiwe. Futhi, qinisekisa ukugcinwa okufanele kanye nokulinganiswa kwethuluzi ukuze uthole imiphumela enembile nethembekile.