I-Gene Electroporator GP-3000
Imininingwane
| Imodeli | GP-3000 |
| I-Pulse Form | Ukubola kwe-Exponential kanye ne-Square Wave |
| Okukhipha amandla kagesi aphezulu | 401-3000V |
| Okukhipha i-voltage ephansi | 50-400V |
| I-high voltage capacitor | 10-60μF ku-1μF izinyathelo (10μF, 25μF, 35μF, 50μF, 60μF kuyanconywa) |
| I-voltage capacitor ephansi | 25-1575μF kuzinyathelo ezingu-1μF (kunconywa izinyathelo ezingu-25μF) |
| I-Parallel resistor | 100Ω-1650Ω kuzinyathelo ezingu-1Ω (kunconyiwe okungu-50Ω) |
| Ukunikezwa kwamandla kagesi | 100-240VAC50/60HZ |
| Isistimu esebenzayo | Ukulawula ikhompuyutha encane |
| Isikhathi njalo | nge-RC isikhathi esingaguquki, esingaguquguquka |
| Isisindo sengqikithi | 4.5kg |
| Ubukhulu bephakheji | 58x36x25cm |
Incazelo
I-cell electroporation iyindlela ebalulekile yokwethula ama-macromolecules angaphandle njenge-DNA, i-RNA, i-siRNA, amaprotheni, nama-molecule amancane ngaphakathi kolwelwesi lwamaseli.
Ngaphansi kwethonya lenkambu kagesi eqinile okwesikhashana, i-membrane yeseli esixazululweni izuza ukuvuthwa okuthile. Izinto ezikhokhiswayo zangaphandle zingena kulwelwesi lweseli ngendlela efana ne-electrophoresis. Ngenxa yokumelana okuphezulu kwe-phospholipid bilayer ye-membrane yeseli, ama-voltage e-bipolar akhiqizwa inkambu yamanje kagesi yangaphandle athwalwa ulwelwesi lwamaseli, futhi i-voltage esatshalaliswa ku-cytoplasm inganakwa, cishe ingabibikho okwamanje ku-cytoplasm, ngaleyo ndlela futhi inqume ubuthi obuncane ebangeni elijwayelekile lenqubo ye-electrophoresis.
Isicelo
Ingasetshenziselwa i-electroporation ukudlulisa i-DNA kumaseli anekhono, amaseli ezitshalo nawezilwane, namaseli emvubelo. Okufana ne-electroporation yamagciwane, imvubelo, nezinye izinto ezincane eziphilayo, ukudluliswa kwamangqamuzana ezilwane ezincelisayo, nokudluliselwa kwezicubu zezitshalo nama-protoplasts, ukuxutshwa kwamaseli kanye nokwethulwa kwezakhi zofuzo, ukwethulwa kwezakhi zofuzo zokulebula nezinjongo zezinkomba, ukwethulwa kwezidakamizwa, amaprotheni, amasosha omzimba, namanye ama-molecule ukuze atadishe ukwakheka nokusebenza kwamangqamuzana.
Isici
• Ukusebenza kahle okuphezulu: isikhathi esifushane sokuguqulwa, izinga eliphezulu lokuguqulwa, ukuphindaphinda okuphezulu;
• Isitoreji esihlakaniphile: singagcina imingcele yokuhlola, elungele abasebenzisi ukuyisebenzisa;
• Ukulawula okunembayo: ukukhishwa kwe-pulse okulawulwa yi-microprocessor;Ø
• Ukubukeka okuhle: umklamo ohlanganisiwe wawo wonke umshini, isibonisi esinembile, ukusebenza okulula.
FAQ
Q: Iyini iGene Electroporator?
A: I-Gene Electroporator iyithuluzi elisetshenziselwa ukwethula izinto zofuzo zangaphandle, njenge-DNA, i-RNA, namaphrotheni, kumaseli ngenqubo ye-electroporation.
Q: Yiziphi izinhlobo zamaseli ezingaqondiswa ngeGene Electroporator?
A: I-Gene Electroporator ingasetshenziswa ukwethula izakhi zofuzo ezinhlotsheni ezihlukahlukene zamaseli okuhlanganisa amagciwane, imvubelo, amaseli ezitshalo, amaseli ezincelisayo, nezinye izinto ezincane eziphilayo.
Q: Yiziphi izinhlelo zokusebenza eziyinhloko zeGene Electroporator?
A:
• I-Electroporation yamagciwane, imvubelo, namanye amagciwane: Okokuguqulwa kofuzo kanye nezifundo zokusebenza kwezakhi zofuzo.
• Ukudluliselwa kwamaseli ezilwane ezincelisayo, izicubu zezitshalo, nama-protoplasts: Ukuze kuhlaziywe isisho sezakhi zofuzo, i-genomics esebenzayo, nobunjiniyela bofuzo.
• Ukuhlanganiswa kwamaseli kanye nesingeniso sokuhlanganiswa kwezakhi zofuzo: Ngokwenza amaseli ayingxube kanye nokwethulwa kofuzo oluhlanganisiwe.
• Ukwethulwa kofuzo lomaka: Okokulebula nokulandelela ukuvela kofuzo kumaseli.
• Ukwethulwa kwezidakamizwa, amaprotheni, namasosha omzimba: Ukuze kuphenywe ukwakheka kweseli nokusebenza, ukulethwa kwezidakamizwa, kanye nezifundo zokusebenzisana kwamaprotheni.
Q: Isebenza kanjani iGene Electroporator?
A: I-Gene Electroporator isebenzisa i-pulse kagesi emfushane, ene-voltage ephezulu ukudala izimbotshana zesikhashana kulwelwesi lweseli, okuvumela ama-molecule angaphandle ukuthi angene kuseli. I-membrane yeseli iqala kabusha ngemva kokushaya kukagesi, ibambe ama-molecule angenisiwe ngaphakathi kweseli.
Q: Yiziphi izinzuzo zokusebenzisa i-Gene Electroporator?
A:Ukuphindaphinda okuphezulu nokusebenza kahle, ukusebenza kalula: Inqubo elula nesheshayo, ukulawulwa komthamo, akukho toxicity ye-genotoxicity: Ukulimala okungaba khona okuncane kofuzo lweseli.
Q: Ingabe iGene Electroporator ingasetshenziswa kuzo zonke izinhlobo zokuhlolwa?
A: Nakuba i-Gene Electroporator inemisebenzi eminingi, ukusebenza kahle kwayo kungahluka kuye ngohlobo lweseli kanye nezinto zofuzo ezethulwayo. Kubalulekile ukukhulisa izimo zokuhlola ngakunye.
Q: Yikuphi ukunakekelwa okukhethekile okudingekayo ngemva kokwethulwa?
IMP: Ukunakekelwa kwangemva kokwethulwa kungase kuhlanganise ukufukamela amaseli endaweni yokutakula ukuze awasize alungise futhi aqalise kabusha imisebenzi evamile. Ukucaciswa kungahluka kuye ngohlobo lweseli nokuhlolwa.
Q: Ingabe kukhona ukukhathazeka ngokuphepha ngokusebenzisa iGene Electroporator?
IMP: Izinqubo ezijwayelekile zokuphepha zaselabhorethri kufanele zilandelwe. I-Gene Electroporator isebenzisa amandla kagesi aphezulu, ngakho-ke ukuphatha okufanele kanye nezinqubo zokuphepha kufanele kulandelwe ukuze kuvinjelwe izingozi zikagesi.
