I-Gel electrophoresis isebenzisa ukushaja okuhle nokubi ukuhlukanisa izinhlayiya ezishajiwe. Izinhlayiya zingashajwa kahle, zibe negetive, noma zibe maphakathi. Izinhlayiya ezishajwayo zikhangwa ngamashaji aphikisanayo: Izinhlayiya ezishajwe kahle zikhangwa ukushajwa okungekuhle, futhi izinhlayiya ezinecala elingelihle zikhangwa amanani avumayo.Ngenxa yokuthi amashaji aphambene ayaheha, singakwazi ukuhlukanisa izinhlayiya sisebenzisa isistimu ye-electrophoresis. Nakuba uhlelo lwe-electrophoresis lungabukeka luyinkimbinkimbi kakhulu, empeleni lulula kakhulu. Amanye amasistimu angase ahluke kancane; kodwa, zonke zinalezi zingxenye ezimbili eziyisisekelo:Power Supply and Electrophoresis Chamber. Sinikeza kokubili i-electrophoresis power supply kanye ne-electrophoresis chamber/tank. Sinemodeli ehlukene ye-electrophoresis ozikhethele yona. Kokubili i-electrophoresis eqondile kanye ne-electrophoresis evundlile kunikezwa ngosayizi abahlukene bejeli kungenziwa njengemfuneko yakho yokuhlola.