I-DYCP-31DN Comb 13/6 imithombo (1.0mm)

Isistimu ye-DYCP-31DN iwuhlelo lwe-electrophoresis oluvundlile.Ku-electrophoresis yejeli evundlile, ijeli ijikijelwa ngomumo ovundlile bese icwiliswa ku-buffer egijima ngaphakathi kwebhokisi lejeli. Ibhokisi lejeli lihlukaniswe izingxenye ezimbili, nejeli ye-agarose ehlukanisa amabili. Njengoba kushiwo ngaphambili, i-anode itholakala ekugcineni, kuyilapho i-cathode itholakala ngakolunye. Ibhafa esebenzayo ye-ionic ivumela ukuthi kudalwe i-gradient yokushaja uma kusetshenziswa i-current. Ngaphezu kwalokho, i-buffer isebenza ukupholisa ijeli, eshisa njengoba kufakwa inkokhiso. I-buffer esebenzayo ivamise ukujikeleziswa kabusha ukuze kuvinjwe ukuthambekela kwe-pH ukuthi yakheke. Sinosayizi ohlukene wamakamu okufanele sisetshenziswe.Amakamu ahlukene enza lolu hlelo oluvundlile lwe-electrophoresis lulungele noma yikuphi ukufakwa kwejeli ye-agarose okuhlanganisa i-submarine electrophoresis, i-electrophoresis esheshayo enamasampuli amancane, i-DNA, i-submarine electrophoresis, ukuhlonza, ukuhlukanisa nokulungisa i-DNA, kanye ukukala isisindo samangqamuzana.

Ngesikhathi se-electrophoresis, kwakhiwa ijeli kuthreyi yokuphonsa. Ithreyi liqukethe "imithombo" emincane ebamba izinhlayiya ofisa ukuzihlola. Ama-microliter amaningana (µL) esixazululo esiqukethe izinhlayiya ofisa ukuzihlola alayishwa ngokucophelela emithonjeni. Khona-ke, i-buffer, eqhuba amandla kagesi, ithululelwa ekamelweni le-electrophoresis. Okulandelayo, ithreyi yokuphonsa, equkethe izinhlayiya, ifakwa ngokucophelela ekamelweni futhi icwiliswe ku-buffer. Ekugcineni, igumbi livaliwe futhi umthombo wamandla uvuliwe. I-anode ne-cathode, edalwe amandla kagesi, iheha izinhlayiya ezikhokhiswa ngokuphambene. Izinhlayiya zihamba kancane kujeli zibheke kwelinye icala. Amandla avaliwe, futhi ijeli iyakhishwa futhi ihlolwe.